Stabilization is the most important remediation mechanisms for sediment polluted heavy metals. However, little research has been done on the identification of microenvironmental response and internal correlation, as well as synergistic mechanisms during heavy metal remediation. This study aims to investigate the inner response mechanisms of microenvironment after the lead (Pb) are gradually stabilized in sediment. An eco-friendly amendment strategy which firstly used 100% biodegradable sophorolipids (SOP) to modify chlorapatite (ClAP) for the fabrication of SOP@nClAP was applied in this study. The stabilization efficiency of Pb was significantly improved by SOP@nClAP compared with ClAP. Most importantly, the high-throughput sequencing showed that the dominant species in the sediment changed with the stabilization of Pb. The decrease of Proteobacteria and increase of Firmicutes, especially the Sedimentibacter within the phylum Firmicute directly suggested that large amounts of Pb were stabilized. This research is not only devoted to stabilize Pb in sediment by eco-friendly amendment strategy, but also keep a watchful eye on microenvironment response mechanisms during the Pb stabilization in sediment. Therefore, this study lays a foundation for the future application of more heavy metal amendment strategies in the sediment environment and improves the possibility of large-scale site amendment.

稳定化是沉积物污染重金属的最重要补救机制。然而，关于微环境响应和内部相关性的鉴定以及重金属修复过程中的协同机制的研究很少。本研究旨在研究沉积物中铅（Pb）逐渐稳定后微环境的内部响应机制。本研究采用了一种生态友好的修饰策略，该策略首先使用100％可生物降解的槐糖脂（SOP）来改性氯磷灰石（ClAP）以制造SOP @ nClAP。与ClAP相比，SOP @ nClAP显着提高了Pb的稳定效率。最重要的是，高通量测序表明，沉积物中的优势物种随着Pb的稳定而变化。减少变形菌门和增加厚壁菌门，特别是Sedimentibacter的内门厚壁菌门直接建议，大量的Pb被稳定化。这项研究不仅致力于通过生态友好的修正策略来稳定沉积物中的Pb，而且还密切关注沉积物中Pb稳定过程中的微环境响应机制。因此，本研究为将来在沉积物中环境中采用更多的重金属修正策略奠定了基础，并提高了大规模场地修正的可能性。