Sugarcane biomasses, such as bagasse and straw, are abundant and represent renewable sources for the production of molecules of economic interest, such as xylan. However, the solubilization methods for this macromolecule face the material recalcitrance and, consequently, need a higher amount of chemicals. This study brings as the fractioning of biomass for xylan extraction, aiming to verify how the recalcitrance of different segments of the plant may interfere with the process of xylan solubilization. Hydrogen peroxide was applied in the xylan solubilization starting from the bagasse, moving on to the leaf and stem, which was fractionated in internode, node, and external fraction (which contains epidermis). The solubilized xylan was evaluated as a substrate for the determination of the enzymatic activity of xylanase. The solubilized xylan was monitored for its solubility and for impurities, such as salt and residual lignin content. The results of xylan solubilization with 6 % of hydrogen peroxide were 86.28, 65.39, 76.78, 97.95, 96.48 %, successively for bagasse, internode, node, external fraction, and leaf. With the application of 3% peroxide, the yield decreased to lower than 30 %, except for the internode and the leaf, which showed 47.85 and 46.15 %, respectively. The RMN analysis suggested similarity among the xylan samples solubilized from the different sugarcane anatomic fractions. The highest enzymatic activities were found with the solubilized xylan, instead of the commercial one. The solubility and purity tests (low salt content) indicate that the solubilized xylan presented characteristics similar to commercial xylan or even better than it.

甘蔗生物质，例如甘蔗渣和稻草，含量丰富，是生产具有经济意义的分子（如木聚糖）的可再生资源。然而，用于该大分子的增溶方法面对材料的难降解性，因此需要更多量的化学物质。这项研究将生物质分馏出来，以提取木聚糖，旨在验证植物不同部位的顽固性如何干扰木聚糖的增溶过程。从甘蔗渣开始，将过氧化氢用于木聚糖增溶，然后移至叶和茎，将其在节间，节和外部级分（包含表皮）中分馏。评价溶解的木聚糖作为确定木聚糖酶酶活性的底物。监测溶解的木聚糖的溶解度和杂质，例如盐和残留的木质素含量。用甘蔗渣，节间，节，外部分和叶依次用6％的过氧化氢溶解木聚糖的结果是86.28％，65.39％，76.78％，97.95％，96.48％。施用3％的过氧化物后，除节间和叶片（分别为47.85％和46.15％）外，产量均降至30％以下。RMN分析表明，从不同甘蔗解剖级分中溶解的木聚糖样品之间具有相似性。发现溶解的木聚糖而不是商业木聚糖具有最高的酶促活性。溶解度和纯度测试（低盐含量）表明，溶解的木聚糖呈现出类似于商品木聚糖的特性，甚至比其更好。