In 2018 virus-like symptoms, typical of polerovirus infection were observed in several oilseed rape crops in northern Greece. In order to identify the etiological agent of these symptoms a polerovirus-generic RT-PCR assay was applied. Sequencing of the amplicons revealed the presence of virus isolates genetically close to turnip yellows virus (TuYV). Further molecular characterization of the near complete genome of ‘1–2’, ‘Geo1’, ‘Geo7’ and ‘Geo15’ isolates revealed that they share > 96% nt identity with various TuYV sequences. On the other hand, the fifth, characterized isolate from oilseed rape, termed ‘1–1’, showed higher sequence similarity to brassica yellows virus (BrYV) regarding the 5′ part of the complete coding sequence, whereas the 3′ part was closely related to TuYV isolates. A recombination analysis using RDP indicated the presence of a putative breakpoint (nucleotide position 2964) in ‘1–1’ genome and it is proposed that the virus isolate ‘1–1’ might be an interspecies recombinant between BrYV and TuYV. To our knowledge, this is the first time that the complete coding sequences of Greek TuYV isolates have been determined and the first detection of a BrYV/TuYV recombinant isolate infecting oilseed rape in Greece.

2018 年，在希腊北部的几种油菜作物中观察到了典型的病毒样症状。为了鉴定这些症状的病原体，应用了极病毒属 RT-PCR 测定法。扩增子的测序显示存在基因上与萝卜黄病毒 (TuYV) 接近的病毒分离株。对“1-2”、“Geo1”、“Geo7”和“Geo15”分离株的近乎完整基因组的进一步分子表征表明，它们与各种 TuYV 序列具有 > 96% 的 nt 同一性。另一方面，从油菜中分离出的第五个特征分离物，称为“1-1”，在完整编码序列的 5' 部分与芸苔黄病毒 (BrYV) 显示出更高的序列相似性，而 3' 部分与与 TuYV 分离株相关。使用 RDP 进行的重组分析表明，'1-1' 基因组中存在一个假定的断点（核苷酸位置 2964），并且提出病毒分离物“1-1”可能是 BrYV 和 TuYV 之间的种间重组。据我们所知，这是首次确定希腊 TuYV 分离株的完整编码序列，并首次在希腊检测到感染油菜的 BrYV/TuYV 重组分离株。