Single nucleotide polymorphisms (SNPs), known as the third-generation molecular markers, are becoming popular in molecular ecology studies. The rapid development of next generation sequencing technologies allow identifying large numbers of SNP markers that can be used in conservation genetics. Based on the genome resequencing dataset of Crested ibis (Nipponia nippon), we developed 31 polymorphic SNP markers using the restriction-site associated DNA (RAD) sequencing. The minor allele frequency per locus varied from 0.176 to 0.485, while the observed and expected heterozygosity varied from 0.118 to 0.794 and from 0.295 to 0.507, respectively. Three loci showed significant departures from Hardy–Weinberg equilibrium. These SNP markers could be useful in future population genetic analysis of Crested ibis.

单核苷酸多态性（SNP），被称为第三代分子标记，在分子生态学研究中正变得越来越流行。下一代测序技术的飞速发展使得能够鉴定出可用于保护遗传学的大量SNP标记。基于朱rest（Nipponia nippon）的基因组重测序数据集，我们使用限制性位点相关DNA（RAD）测序开发了31种多态性SNP标记。每个基因座的次要等位基因频率从0.176到0.485，而观察到的和预期的杂合度分别从0.118到0.794和0.295到0.507。三个基因座显示出与Hardy-Weinberg平衡的显着偏离。这些SNP标记可能对凤头朱鹭的未来种群遗传分析有用。