Contamination and degradation are known challenges for reliable genotyping, since they can cause, among other problems, false microsatellite profiles. In this study we described a method to decrease the proportion of false microsatellite profiles from fish scale samples of endangered allis shads (Alosa alosa) from a reintroduction program, where cross-contamination with DNA from other individuals and potentially degradation of samples occurred. To maximize the portion of reliably measurable results, we modified and combined two known approaches—thresholds used in forensic DNA analyses and a multiple-tubes approach. This combined approach increased reliable microsatellite profiles compared with single approaches. The forensic thresholds and the multiple-tubes approach increased the measurable results from 55 to 67% and 75%, respectively, whereas the combined approach accomplished an increase to 90%. This illustrates the potential of the combined approach for other studies with comparable problems or sample material.

污染和降解是可靠基因分型的已知挑战，因为除其他问题外，污染和降解会导致错误的微卫星轮廓。在这项研究中，我们描述了一种方法，可从濒临灭绝的艾利斯鱼（Alosa alosa）的鱼鳞样品中减少假微卫星轮廓的比例）的重新导入程序，其中发生了与其他个体DNA的交叉污染和样品的潜在降解。为了最大限度地提高可靠的可测量结果的比例，我们修改并组合了两种已知的方法-法医DNA分析中使用的阈值和多管方法。与单一方法相比，这种组合方法增加了可靠的微卫星轮廓。法医阈值和多管法分别将可测量的结果从55％增加到67％和75％，而组合法则将可测量的结果增加到90％。这说明了结合方法在其他具有类似问题或样品材料的研究中的潜力。