Sodium-potassium ATPase (Na, K-ATPase) is an integral protein universally distributed in the plasma membrane of all eukaryotic cells. It regulates trans-epithelial sodium ion transport, muscle contraction, nerve excitability, secretary functions and intracellular signaling. We investigated the mechanism of vasorelaxation effect of Eugenol, Curcumin and Nanocurcumin in the middle uterine artery (MUA) of goat to evaluate their possible therapeutic potential in hypertension during pregnancy or pre-eclampsia. The UA rings were isometrically contracted by K⁺-free MKHS to plateau and relaxed with KCl (10 μM-10 mM) following standard protocol. The K⁺ -vasorelaxation response (VR) was taken either in presence of ouabain with or without Eugenol/Curcumin/Nanocurcumin (10 μM). R_max (maximum % vasorelaxation) of K⁺-induced VR-curve elicited in UA rings was reduced (from 6.90% and 26.55%) to 4-5% in presence of Ouabain in both NP and P goats. R_max obtained from KVR curve elicited in UA rings were augmented in presence of Eugenol (NP: 16.22%, P: 42.67%) or Curcumin (NP:21.23%, P:35.49%) or Nanocurcumin (NP:6.91%, P:34.88%). K⁺ vasorelaxation was inhibited in presence of Ouabain and Eugenol/ Curcumin/Nanocurcumin (R_max: 6.11%, 9.18%, 4.11% in non-P, respectively) and (R_max: 5.40%, 3.39%, 6.00% in P, respectively). The results indicated that (i) there is increased function of α₁- Na⁺, K⁺-ATPase in UA of P goats (ii) Eugenol, Curcumin and Nano-curcumin differentially augmented the function and expression of α₁ isoenzymes of sodium pump in UA of non-P and P goats. Thus, the polyphenols potentially activate α₁ isoenzymes of Na⁺-K⁺-ATPase and could possibly be useful as therapeutic in hypertension during pregnancy.

中文翻译：

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Na +，K + -ATPase活性差异介导的山羊Cap中动脉对丁香酚，姜黄素和纳米姜黄素的血管舒张作用

钠钾ATP酶（Na，K - ATPase）是一种普遍存在于所有真核细胞质膜中的不可或缺的蛋白质。它调节跨上皮钠离子运输，肌肉收缩，神经兴奋性，秘书功能和细胞内信号传导。我们研究了丁香酚，姜黄素和纳米姜黄素对山羊子宫中动脉（MUA）血管舒张作用的机制，以评估其在妊娠或先兆子痫中对高血压的治疗潜力。UA环通过无K ^+的MKHS等轴测收缩至平台，并按照标准方案用KCl（10μM-10mM）松弛。K ⁺在有或没有丁子香酚/姜黄素/纳米姜黄素（10μM）的哇巴因存在下进行血管舒张反应（VR）。在NP和P山羊中，在存在Ouabain的情况下，在UA环中引起的K ⁺诱导的VR曲线的R _max（最大血管舒张百分比）从6.90％和26.55％降低到4-5％。ř_最大值从在UA环引起KVR曲线得到丁子香酚（NP：16.22％，P：42.67％）的存在下，增加或姜黄素（NP：21.23％，P：35.49％）或纳米姜黄素（NP：6.91％，P： 34.88％）。ķ ⁺血管舒张被抑制在哇巴因和丁子香酚/姜黄素/纳米姜黄素（R的存在_最大：分别6.11％，9.18％，在非P 4.11％，）和（R_最大：P分别为5.40％，3.39％和6.00％）。结果表明，α（i）的存在增加的函数₁ -的Na ⁺，K ⁺ -ATP酶P中山羊UA（II）丁子香酚，姜黄素和纳米姜黄素差异增强的α的功能和表达₁钠泵同工酶在非P和P山羊的UA中。因此，多酚潜在激活α _1种的Na同工酶⁺ -K ⁺ -ATP酶和妊娠期间也可能会被作为高血压的治疗是有用的。