The aim of this study was to determine the cytotoxic effect of black mulberry fruit extract (Morus nigra L.) on human breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. The extract prepared from mulberry (M. nigra) fresh fruit was applied to cancer cell lines in 5 different v/v concentrations (10%, 4%, 2%, 1.33% and 1%) for 72 h. At the end of the incubation period, cell viability was determined by MTT [3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide] assay. Results were compared with negative and positive control groups. The effect of mulberry (M. nigra) fruit extract on cell viability was determined depending on the concentration applied; extract showed a significant cytotoxic effect on PC3 cell line at the 10% concentration compared to the control group, but not detected any effect at 4%, 2%, 1.33% and 1% concentrations. In the MDA-MB-231 cell line, there was a high cytotoxic effect at a concentration of 10% compared to the control group, while a lower effect was observed at 4%, 2%, 1.33% and 1% concentrations. The data obtained from the study showed that extract prepared from black mulberry fruit has cytotoxic effect especially against breast cancer cells.

中文翻译：

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黑桑果的细胞毒性作用研究

这项研究的目的是确定黑桑树果实提取物（桑属黑桑树）对人乳腺癌（MDA-MB-231）和前列腺癌（PC3）细胞系的细胞毒性作用。从桑树（黑果）新鲜水果中提取的提取物以5种不同的v / v浓度（10％，4％，2％，1.33％和1％）应用于癌细胞系72小时。在孵育期结束时，通过MTT [3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物]测定来确定细胞活力。将结果与阴性和阳性对照组进行比较。桑（效果M.黑）根据所用浓度确定水果提取物对细胞活力的影响；提取物在10％浓度下与对照组相比对PC3细胞系具有明显的细胞毒性作用，但在4％，2％，1.33％和1％浓度下未检测到任何作用。在MDA-MB-231细胞系中，与对照组相比，浓度为10％时有较高的细胞毒性作用，而浓度为4％，2％，1.33％和1％时则观察到较低的作用。从研究中获得的数据表明，从黑桑果中提取的提取物具有细胞毒性作用，尤其是对乳腺癌细胞。