DNA-based nanomachines have aroused tremendous interest because of their potential applications in bioimaging, biocomputing, and diagnostic treatment. Herein, we constructed a novel exonuclease III-propelled and signal-amplified stochastic DNA walker that autonomously walked on a spherical particle-based 3D track through a burnt-bridge mechanism, during which nanosurface energy transfer (NSET) occurred between the fluorescent dye modified on hairpin DNA and the surface of gold nanoparticles (AuNPs). As a proof of concept, this stochastic DNA walker achieves prominent detection performance of HIV DNA in the range of 0.05–1.2 nM with a detection limit of 12.7 pM and satisfactory recovery in blood serum, showing high promise in biosensing applications with complicated media.

中文翻译：

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纳米表面能量转移表明Exo III推动了随机3D DNA Walker用于HIV DNA检测

基于DNA的纳米机器因其在生物成像，生物计算和诊断治疗中的潜在应用而引起了极大的兴趣。在这里，我们构建了一种新型的由核酸外切酶III推动和信号放大的随机DNA步行器，该步行器通过燃烧桥机制在球形粒子基3D轨道上自主行走，在此过程中，在经修饰的荧光染料之间发生了纳米表面能量转移（NSET）。发夹DNA和金纳米颗粒（AuNPs）的表面。作为概念的证明，该随机DNA Walker在0.05–1.2 nM的范围内具有出色的HIV DNA检测性能，检测极限为12.7 pM，并且血清中的回收率令人满意，在复杂介质的生物传感应用中显示出很高的希望。