The new technology of high‐throughput single‐cell RNA sequencing (10 × scRNA‐seq) was developed recently with many advantages. However, it was not commonly used in farm animal research. There are few reports for the gene expression of goat ovarian follicle granulosa cells (GCs) during different developmental stages. In the current investigation, the gene expression of follicle GCs at different stages from two populations of Ji'ning grey goats: high litter size (HL; ≥3/L; 2 L) and low litter size (LL; ≤2 /L; 2 L) were analysed by scRNA‐seq. Many GC marker genes were identified, and the pseudo‐time showed that GCs developed during the time course which reflected the follicular development and differentiation trajectory. Moreover, the gene expression difference between the two populations HL versus LL was very clear at different developmental stages. Many marker genes differentially expressed at different developmental stages. ASIP and ASPN were found to be highly expressed in the early stage of GCs, INHA, INHBA, MFGE8 and HSD17B1 were identified to be highly expressed in the growing stage of GCs, while IGFBP2, IGFBP5 and CYP11A1 were found to be highly expressed in late stage. These marker genes could be used as reference genes of goat follicle GC development. This investigation for the first time discovered the gene expression patterns in goat follicle GCs in high‐ or low‐fertility populations (based on litter size) by scRNA‐seq which may be useful for uncovering the oocyte development potential.

中文翻译：

---

来自不同生育山羊的卵巢卵泡颗粒细胞的 scRNA-seq 显示出不同的表达模式

最近开发的高通量单细胞RNA测序（10×scRNA-seq）新技术具有许多优点。然而，它在农场动物研究中并不常用。关于山羊卵巢卵泡颗粒细胞（GCs）在不同发育阶段的基因表达的报道很少。在目前的调查中，济宁灰山羊两个种群不同阶段卵泡GCs的基因表达：高产仔数（HL；≥3/L；2 L）和低产仔数（LL；≤2/L； 2 L) 通过 scRNA-seq 进行分析。鉴定了许多GC标记基因，假时间表明GC在反映卵泡发育和分化轨迹的时间过程中发育。而且，HL 和 LL 两个种群的基因表达差异在不同发育阶段非常明显。许多标记基因在不同发育阶段差异表达。ASIP和ASPN在GCs早期高表达，INHA、INHBA、MFGE8和HSD17B1在GCs生长期高表达，而IGFBP2、IGFBP5和CYP11A1在晚期高表达。阶段。这些标记基因可作为山羊卵泡GC发育的参考基因。本研究首次通过 scRNA-seq 发现了高或低生育率种群（基于产仔数）中山羊卵泡 GC 的基因表达模式，这可能有助于揭示卵母细胞发育潜力。许多标记基因在不同发育阶段差异表达。ASIP和ASPN在GCs早期高表达，INHA、INHBA、MFGE8和HSD17B1在GCs生长期高表达，IGFBP2、IGFBP5和CYP11A1在GCs后期高表达。阶段。这些标记基因可作为山羊卵泡GC发育的参考基因。本研究首次通过 scRNA-seq 发现了高或低生育率种群（基于产仔数）中山羊卵泡 GC 的基因表达模式，这可能有助于揭示卵母细胞发育潜力。许多标记基因在不同发育阶段差异表达。ASIP和ASPN在GCs早期高表达，INHA、INHBA、MFGE8和HSD17B1在GCs生长期高表达，而IGFBP2、IGFBP5和CYP11A1在晚期高表达。阶段。这些标记基因可作为山羊卵泡GC发育的参考基因。本研究首次通过 scRNA-seq 发现了高或低生育率种群（基于产仔数）中山羊卵泡 GC 的基因表达模式，这可能有助于揭示卵母细胞发育潜力。发现IGFBP5和CYP11A1在晚期高表达。这些标记基因可作为山羊卵泡GC发育的参考基因。本研究首次通过 scRNA-seq 发现了高或低生育率种群（基于产仔数）中山羊卵泡 GC 的基因表达模式，这可能有助于揭示卵母细胞发育潜力。发现IGFBP5和CYP11A1在晚期高表达。这些标记基因可作为山羊卵泡GC发育的参考基因。本研究首次通过 scRNA-seq 发现了高或低生育率种群（基于产仔数）中山羊卵泡 GC 的基因表达模式，这可能有助于揭示卵母细胞发育潜力。