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Assessment of aflatoxin B1 in animal feed and aflatoxin M1 in raw milk samples of different species of milking animals from Punjab, Pakistan
Journal of Food Safety ( IF 1.9 ) Pub Date : 2021-02-23 , DOI: 10.1111/jfs.12893
Muhammad Waqas 1 , Wajeeha Pervaiz 1 , Khalid Mahmood Zia 2 , Shahzad Zafar Iqbal 1
Affiliation  

The research was designed to investigate the amount of aflatoxin B1 (AFB1) in animal feed (corn, wheat bran, peanut cake, soybean meal, and cotton seed meal) and aflatoxin M1 (AFM1) in raw milk of animals (cow milk, buffalo milk, goat milk and sheep milk), from Punjab, Pakistan collected March 2017 till February 2018. The samples were examined with liquid chromatography, with a fluorescence detector. The findings demonstrated that 126 (65.3%) out of 193 samples of animal feed were found positive with AFB1, and 88 (45.6%) samples have levels higher than 5 μg/kg; positive samples are those whose levels of AFM1 ≥ LOD. The highest average amount of AFB1 was found in mustard oil cake meal (25.0 ± 4.5 μg/kg). Out of 278 samples of milk, 134 (48.2%) were found positive with AFM1, and 48 (17.3%) samples have levels of AFM1 higher than the European Union legal limit (i.e., 50 ng/L). The highest mean level of 70.5 ± 10.5 ng/L was found in raw milk of buffalo. The levels of AFM1 in milk from the urban region versus suburb region showed a significant difference (at α 0.05) except sheep milk samples, which presented a significant difference at α 0.01. Samples, 65 (64.4%) out of 140 from the urban region, and 69 (50%) from the suburb region were found positive with AFM1 in milk samples. The amount of AFB1 in animal feed and AFM1 in milk samples are comparatively high and could pose health hazards for local consumers.

中文翻译:

评估来自巴基斯坦旁遮普省的不同种类挤奶动物的动物饲料中的黄曲霉毒素 B1 和生奶样品中的黄曲霉毒素 M1

本研究旨在调查动物饲料(玉米、麦麸、花生饼、豆粕和棉籽粕)中黄曲霉毒素 B 1 (AFB 1 ) 和动物原奶中黄曲霉毒素 M 1 (AFM 1 )的含量(牛奶、水牛奶、山羊奶和绵羊奶),来自巴基斯坦旁遮普邦,采集于 2017 年 3 月至 2018 年 2 月。样品采用液相色谱法和荧光检测器进行检测。结果表明,在 193 个动物饲料样品中,有 126 个(65.3%)被发现 AFB 1呈阳性,88 个(45.6%)样品的水平高于 5 μg/kg;阳性样本是那些 AFM 1  ≥ LOD水平的样本。AFB 1的最高平均量在芥末油饼粕中发现(25.0 ± 4.5 μg/kg)。在 278 个牛奶样品中,134 个 (48.2%) 被发现 AFM 1呈阳性,48 个 (17.3%) 样品的 AFM 1水平高于欧盟法律限制(即 50 ng/L)。在水牛生奶中发现的最高平均水平为 70.5 ± 10.5 ng/L。来自城市地区和郊区的牛奶中 AFM 1的水平显示出显着差异(α 0.05),除了羊奶样品,其在 α 0.01 处存在显着差异。来自城市地区的 140 个样本中的 65 个(64.4%)和来自郊区的 69 个(50%)样本在牛奶样品中被发现 AFM 1呈阳性。动物饲料中 AFB 1和 AFM 1的含量 牛奶样品中的含量相对较高,可能对当地消费者的健康构成危害。
更新日期:2021-02-23
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