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Evaluation of yeast isolates from kimchi with antagonistic activity against green mold in citrus and elucidating the action mechanisms of three yeast: P. kudriavzevii, K. marxianus, and Y. lipolytica
Postharvest Biology and Technology ( IF 6.4 ) Pub Date : 2021-02-23 , DOI: 10.1016/j.postharvbio.2021.111495
Kottoh Isaac Delali , Ou Chen , Wenjun Wang , Lanhua Yi , Lili Deng , Kaifang Zeng

Many citrus farmers are deprived of their profits as a result of post-harvest losses in citrus production. Diverse microbial environments including fermented foods are necessary to be explored in the quest to get more novel biocontrol yeasts to control post-harvest pathogens in citrus. 90 yeasts were isolated from kimchi in this investigation. Inhibition tests in vitro showed that 10 of the 90 yeasts reduced the development of fungal mycelia by the formation of an inhibition zone. Molecular methods were used to identify the yeast isolates, and they were Pichia sp., Kluyveromyces marxianus, Yarrowia lipolytica, and Issatchenkia orientalis. Three isolates tested in vivo in citrus were able to reduce disease incidence within the range from 18 % to 57 %. The action mechanisms of the three yeasts were studied subsequently by determining the biofilm formation ability of the yeast isolates, screening of yeast isolates for extracellular lytic enzyme activity, evaluating the effect of yeast isolates’ volatile organic compounds (VOCs) on P. digitatum, investigating the competition for nutrients between P. digitatum and yeast isolates, etc. For Pichia kudriavzevii, the most effective one, reduced the incidence of green mold caused by Penicillium digitatum (Pd1 / CECT 20795) by biofilm formation, competition for nutrients, and emitting volatile organic compounds. K. marxianus showed antifungal activity by inducing resistance, and colonization on wound sites. Whereas Y. lipolytica inhibited P. digitatum by adhesion to pathogen mycelia and production of extracellular lytic enzymes. Evidence from this study suggests that some yeast strains isolated from kimchi have the potentials to inhibit green mold in citrus.



中文翻译:

从对柑橘绿霉菌的拮抗活性酵母泡菜分离的评价和阐明3个酵母的作用机制:库德里阿兹威毕赤酵母马克斯克鲁维Y.脂

由于柑桔生产的收获后损失,许多柑桔农民被剥夺了利润。为了获得更多新型的生物控制酵母以控制柑橘收获后的病原体,有必要探索包括发酵食品在内的多种微生物环境。在这项调查中,从泡菜中分离出90种酵母。体外抑制试验表明,90个酵母菌中有10个通过形成抑制区而减少了真菌菌丝体的形成。使用分子方法鉴定酵母分离物,它们是毕赤酵母属。,马尔克斯克鲁维酵母解脂耶氏酵母东方伊萨氏菌。体内测试三种分离株柑橘中的维生素B1能够将疾病发生率降低18%至57%。三个酵母的作用机理进行了通过确定酵母分离株的生物膜形成的能力,酵母分离株为细胞外裂解酶活性的筛选,评估对酵母分离株挥发性有机化合物(VOC)的效果随后研究状青霉,调查为之间的营养物质的竞争状青霉和酵母分离株,等。对于库德里阿兹威毕赤酵母,最有效的一个,降低由绿霉的发生状青霉通过生物膜形成,对营养的竞争,并发射易失性(PD1 / CECT 20795)有机化合物。马克斯克鲁维酵母通过诱导抗性表现出抗真菌活性,并在伤口部位定居。而解脂耶酵母通过粘附至病原体菌丝体和产生细胞外裂解酶来抑制数指疟原虫。这项研究的证据表明,从泡菜中分离出的某些酵母菌株具有抑制柑橘中绿色霉菌的潜力。

更新日期:2021-02-24
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