Cancer has become the leading cause of death for its high incidence and mortality. Early diagnosis and treatment are essential for the inhibition of tumor growth. In this work, A biocompatible, nontoxic, near-infrared fluorescent tumor targeting nanoparticle KHLF(K₅HoLi₂F₁₀) was prepared by a delicate method for the early diagnosis of tumor. The surface functionalization of the nanoparticle endows it with good water solubility and biocompatibility. Covalent binding to folic acid provides it with the ability to target HeLa cells. After modification, this nanoparticle can still maintain high fluorescence intensity and it can be detected in 2 cm thick pork tissue. We carried out biodistribution imaging, organs imaging and tumor targeting imaging experiments to characterize its imaging ability in vivo. The results show that this nanoparticle could accurately accumulate in HeLa tumor and generate significant fluorescence signal, which confirms the nanoparticle has great potential as a efficient biocompatible fluorescent contrast agent for tumor diagnosis.

癌症因其高发病率和高死亡率已成为死亡的主要原因。早期诊断和治疗对于抑制肿瘤生长至关重要。在这项工作中，靶向纳米颗粒KHLF（K ₅ HoLi ₂ F _10）的生物相容性，无毒，近红外荧光肿瘤）是通过精密的方法制备的，用于肿瘤的早期诊断。纳米颗粒的表面功能化使其具有良好的水溶性和生物相容性。与叶酸的共价结合使其具有靶向HeLa细胞的能力。修饰后，该纳米粒子仍可以保持高荧光强度，并且可以在2厘米厚的猪肉组织中检测到。我们进行了生物分布成像，器官成像和肿瘤靶向成像实验，以表征其在体内的成像能力。结果表明，该纳米粒子可以准确地在HeLa肿瘤中蓄积并产生明显的荧光信号，这证明该纳米粒子作为一种高效的生物相容性荧光造影剂具有广阔的潜力，可用于肿瘤诊断。