Abstract

Cultivation of Azotobacter vinelandii D-08 in media with molasses and distillery waste was shown to increase levansaccharase activity and levan biosynthesis. Levansaccharase also exhibited maximal activity when grown in a molasses medium, which correlated with the highest level of the polysaccharide synthesis. An additional fraction of high molecular weight levan (more than 1000 kDa) with a modified molecular structure (reduced contribution of double bond oscillations and decreased molecule hydration) was synthesized by bacteria, which resulted in a higher capacity for the binding of Na, Mg, S, Ca, and Fe in the cultivation medium. Cryogels based on polyvinyl alcohol and levan were capable of efficient maximal absorption of different ions at different times: copper (1 h), zinc (24 h), and cadmium (10 min), radioactive plutonium (4 h), and uranium (168 h). This suggests that they can be used as sorbents for wastewater treatment to eliminate heavy metals and radionuclides.

中文翻译：

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来自葡萄固氮菌的Levan作为重金属和放射性核素生物吸附剂的成分

摘要

葡萄固氮菌的培养D-08在含糖蜜和酒厂废物的培养基中显示出可增加乙酰聚糖酶活性和Levan生物合成。当在糖蜜培养基中生长时，左旋糖酶也表现出最大的活性，这与多糖合成的最高水平相关。细菌合成了另一部分具有修饰的分子结构（减少了双键振荡的作用和减少了分子水合作用）的高分子量levan（大于1000 kDa），从而提高了与Na，Mg，培养基中的S，Ca和Fe。基于聚乙烯醇和levan的冰晶能够在不同时间有效地最大吸收不同离子：铜（1小时），锌（24小时）和镉（10分钟），放射性p（4小时）和铀（168） H）。