The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID₅₀)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

中文翻译：

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实时RT-PCR快速检测H2亚型甲型禽流感病毒的开发与应用

H2 亚型禽流感病毒（禽 IAV）已在家禽中传播，它们有可能感染人类。因此，建立一种快速检测这种亚型的方法至关重要。我们开发了一种 TaqMan 小沟结合剂实时 RT-PCR 测定，该测定涉及基于基质和血凝素基因的保守序列的探针和引物。该测定的检测限低至每次反应一个 50% 鸡蛋感染剂量 (EID ₅₀ )/mL。这种检测方法对检测禽 IAV H2 亚型具有特异性、敏感性和快速性。