Abstract

Recently, Cryptococcus pseudolongus has been reported as a new pathogen of shiitake (Lentinula edodes). However, its pathological properties are not much known. To further understand its impact on the mushroom, we investigated the pathogen’s interactions with the mycelium of shiitake, histopathological properties, host range, and sensitivity to diverse antifungal agents. The strain C. pseudolongus DUCC 4014 inhibited the mycelial growth of L. edodes strain (cultivar Sanjo 701ho) and caused browning in the mycelia confronted with the yeast on PDA. Spray inoculation of the yeast caused an abnormal browning symptom on the cap and/or gills of three shiitake cultivars grown on sawdust media in vinyl bags. Scanning electron microscopic images of the abnormally browned parts of shiitake fruit body illustrated that mushroom tissues were loosed and dispersed in the middle and edge of the cap and the arrangement of basidiospores borne on basidia in the gills was disturbed compared to those of normal shiitake fruit body. Spray inoculation also led to developing abnormal browning on the harvested fruit body, indicating C. pseudolongus could be a problem during mushroom storage. But the yeast was not able to induce abnormal browning on mushrooms of Pleurotus ferulae, Pleurotus fostreatus, and Agaricus bisporus. But it induced browning only on button mushroom (A. bisporus) when they were inoculated after wounding. Tests with 16 kinds of fungicides revealed that the cell growth of C. pseudolongus could be inhibited by benzalkonium chloride at MIC 7 μg/ml and benomyl at MIC 3 μg/ml.

 抽象的

最近，有报道称假长隐球菌是一种新的香菇病原菌。然而，其病理特性尚不清楚。为了进一步了解其对蘑菇的影响，我们研究了病原体与香菇菌丝体的相互作用、组织病理学特性、宿主范围以及对不同抗真菌药物的敏感性。菌株C。假长菌DUCC 4014 抑制香菇菌株（品种 Sanjo 701ho）的菌丝生长，并导致 PDA 上与酵母面对的菌丝体褐变。喷雾接种酵母会导致在乙烯基袋中的锯末培养基上生长的三个香菇品种的菌盖和/或菌褶出现异常褐变症状。香菇子实体异常褐变部分的扫描电镜图像显示，与正常香菇子实体相比，菌盖中部和边缘的蘑菇组织疏松、分散，菌褶中担子上的担子孢子排列受到干扰。 。喷雾接种还导致收获的子实体出现异常褐变，表明C 。假长丝可能是蘑菇储存过程中的一个问题。但酵母无法诱导阿魏侧耳、黄侧耳和双孢蘑菇的蘑菇异常褐变。但只有双孢蘑菇（ A. bisporus ）受伤后接种时才引起褐变。 16种杀菌剂试验表明，苯扎氯铵在MIC 7 μg/ml下和苯菌灵在MIC 3 μg/ml下均能抑制假长假丝线虫细胞的生长。