Abstract

Aim

To validate neuroprotective effect of pectin against neuropathic pain in diabetic rodents.

Material and method

Pectin was isolated and characterised from different sources to validate its neuroprotective effect against T2DM associated neuropathic pain. The antioxidant activity of pectins was done by the DPPH method. Type-2 diabetes mellitus (T2DM) was induced in Wistar albino rats by high-fat diet and high-fat emulsion feeding for 2 weeks followed by a single i.p. of Sterptozotocin in 3rd week. The animals were grouped as positive control and Citrus sinensis (L.) Osbeck peel pectin (CSL-OP) as test group and treated for the next 4 weeks. Body weight and blood glucose were measured up to 8 weeks; however, behavioural assessment was done at the end of 5th to 8th week.

Result

CSL-OP restored the reduced body weight and elevated blood glucose with increased pain threshold and improved walking performance.

Conclusion

CSL-OP prevented progression of early diabetic neuropathy with anti-oxidant activity.

中文翻译：

---

果胶对 T2DM 引起的糖尿病周围神经性疼痛异常性疼痛和痛觉过敏的神经保护作用验证

摘要

目的

验证果胶对糖尿病啮齿动物神经性疼痛的神经保护作用。

材料与方法

从不同来源分离果胶并对其进行表征，以验证其对 T2DM 相关神经性疼痛的神经保护作用。采用DPPH法测定果胶的抗氧化活性。通过高脂饮食和高脂乳剂喂养 2 周，然后在第 3 周单次腹膜内注射链脲佐菌素 (Sterptozotocin)，在 Wistar 白化大鼠中诱导 2 型糖尿病 (T2DM)。将动物分组为阳性对照，将柑橘（L.）Osbeck果胶（CSL-OP）作为测试组，并在接下来的4周内进行治疗。测量体重和血糖长达 8 周；然而，行为评估是在第 5 至 8 周结束时进行的。

结果

CSL-OP 恢复了减轻的体重和升高的血糖，同时增加了疼痛阈值并改善了步行能力。

结论

CSL-OP 具有抗氧化活性，可预防早期糖尿病神经病变的进展。