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Changes in NOTCH1 gene and its regulatory circRNA, hsa_circ_0005986 expression pattern in human gastric adenocarcinoma and human normal fibroblast cell line following the exposure to extremely low frequency magnetic field
Electromagnetic Biology and Medicine ( IF 1.6 ) Pub Date : 2021-02-23 , DOI: 10.1080/15368378.2021.1891092
Fereshteh Mansoury 1 , Nahid Babaei 1 , Soheila Abdi 2 , Maliheh Entezari 3 , Abbas Doosti 4
Affiliation  

ABSTRACT

The effect of an extremely low-frequency magnetic field (ELF-MFs) on the expression levels of NOTCH1 and its regulatory circular RNA (circ-RNA) in gastric cancer has not yet investigated. This study aimed to find the expression changes of NOTCH1 and its regulatory circ-RNA, hsa_circ_0005986, in human gastric adenocarcinoma cell line (AGS) and human normal fibroblast (Hu02) cells fallowing the exposure to discontinuously magnetic flux densities (MFDs) of 0.25, 0.5 ,1 and 2 millitesla (mT) for 18h in comparison to unexposed cells. In addition, the effect of various MFDs on viability of tumor and normal cells was investigated. The cell viability was evaluated by MTT assay. The relative expression of NOTCH1and hsa_circ_0005986 mRNAs was analyzed by quantitative Real-time PCR. The viability of tumor cells was decreased under the exposure of MFs, while the normal cells viability was increased. NOTCH1 was significantly down-regulated in AGS cells and up-regulated in Hu02 cells at all MFDs. The expression changes of NOTCH1 in tumor and normal cells was depended to the MFD of MFs. According to our results, the tumor and normal cells show different behavior at the molecular level in various MFDs in terms of NOTCH1 and hsa_circ_0005986 expression level. Decrease in tumor cell survival following the exposure to ELF-MFs may be the result of decreased in the expression level of NOTCH1 and its Reg-circ-RNA. These magnetic field-reducing effects on cancer cell survival through the change on the expression of genes involved in the proliferation and progression of cancer can be a new key in cancer treatment.



中文翻译:

极低频磁场暴露在人胃腺癌细胞和人正常成纤维细胞系中NOTCH1基因及其调控circRNA、hsa_circ_0005986表达模式的变化

摘要

尚未研究极低频磁场(ELF-MFs)对胃癌中NOTCH1及其调节环状RNA(circ-RNA)表达水平的影响。本研究旨在发现 NOTCH1 及其调控 circ-RNA hsa_circ_0005986 在人胃腺癌细胞系 (AGS) 和人正常成纤维细胞 (Hu02) 细胞中在不连续磁通密度 (MFD) 暴露于 0.25 时的表达变化,与未暴露的细胞相比,0.5 ,1 和 2 毫特斯拉 (mT) 18 小时。此外,研究了各种 MFD 对肿瘤和正常细胞活力的影响。通过MTT测定评估细胞活力。通过定量实时PCR分析NOTCH1和hsa_circ_0005986 mRNA的相对表达。MFs暴露下肿瘤细胞的活力降低,而正常细胞活力增加。在所有 MFD 中,NOTCH1 在 AGS 细胞中显着下调,在 Hu02 细胞中上调。NOTCH1在肿瘤和正常细胞中的表达变化取决于MFs的MFD。根据我们的结果,就NOTCH1和hsa_circ_0005986表达水平而言,肿瘤和正常细胞在各种MFDs的分子水平上表现出不同的行为。暴露于 ELF-MF 后肿瘤细胞存活率降低可能是 NOTCH1 及其 Reg-circ-RNA 表达水平降低的结果。这些通过改变参与癌症增殖和进展的基因表达来降低磁场对癌细胞存活的影响可能是癌症治疗的新关键。在所有 MFD 中,NOTCH1 在 AGS 细胞中显着下调,在 Hu02 细胞中上调。NOTCH1在肿瘤和正常细胞中的表达变化取决于MFs的MFD。根据我们的结果,就NOTCH1和hsa_circ_0005986表达水平而言,肿瘤和正常细胞在各种MFDs的分子水平上表现出不同的行为。暴露于 ELF-MF 后肿瘤细胞存活率降低可能是 NOTCH1 及其 Reg-circ-RNA 表达水平降低的结果。这些通过改变参与癌症增殖和进展的基因表达来降低磁场对癌细胞存活的影响可能是癌症治疗的新关键。在所有 MFD 中,NOTCH1 在 AGS 细胞中显着下调,在 Hu02 细胞中上调。NOTCH1在肿瘤和正常细胞中的表达变化取决于MFs的MFD。根据我们的结果,就NOTCH1和hsa_circ_0005986表达水平而言,肿瘤和正常细胞在各种MFDs的分子水平上表现出不同的行为。暴露于 ELF-MF 后肿瘤细胞存活率降低可能是 NOTCH1 及其 Reg-circ-RNA 表达水平降低的结果。这些通过改变参与癌症增殖和进展的基因表达来降低磁场对癌细胞存活的影响可能是癌症治疗的新关键。根据我们的结果,就NOTCH1和hsa_circ_0005986表达水平而言,肿瘤和正常细胞在各种MFDs的分子水平上表现出不同的行为。暴露于 ELF-MF 后肿瘤细胞存活率降低可能是 NOTCH1 及其 Reg-circ-RNA 表达水平降低的结果。这些通过改变参与癌症增殖和进展的基因表达来降低磁场对癌细胞存活的影响可能是癌症治疗的新关键。根据我们的结果,就NOTCH1和hsa_circ_0005986表达水平而言,肿瘤和正常细胞在各种MFDs的分子水平上表现出不同的行为。暴露于 ELF-MF 后肿瘤细胞存活率降低可能是 NOTCH1 及其 Reg-circ-RNA 表达水平降低的结果。这些通过改变参与癌症增殖和进展的基因表达来降低磁场对癌细胞存活的影响可能是癌症治疗的新关键。

更新日期:2021-02-23
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