ABSTRACT

In this study, an amino-functionalized magnetic silica microsphere material (Fe₃O₄-SiO₂-NH₂) was prepared. Using glutaraldehyde as a cross-linking agent, Trametes versicolor laccase was adsorbed-covalently bonded and immobilized on the material to prepare Laccase @ Fe₃O₄-SiO₂. In addition, the materials were characterized and analysed by SEM, TEM, XRD, FT-IR and VSM. Finally, the thermal inactivation dynamics of immobilized laccase in polar/non-polar/toxic systems and the adsorption and degradation of 2,4-DCP were studied. The results showed that Laccase @ Fe₃O₄-SiO₂ under the optimal conditions (pH 6, temperature 65°C, initial concentration of 2,4-DCP 10 mg/L), the removal rate was as high as 81.6%. Moreover, compared with free laccase, immobilized laccase had good tolerance under low pH and high-temperature conditions, and storage stability was also greatly improved. After repeated use for 7 times, Laccase @ Fe₃O₄-SiO₂ can still maintain 59% removal rate of 2,4-DCP, which gives it the potential for industrial applications.

摘要

本研究制备了一种氨基功能化的磁性二氧化硅微球材料（Fe ₃ O ₄ -SiO ₂ -NH ₂）。以戊二醛为交联剂，将云芝漆酶吸附-共价键固定在材料上，制备了漆酶@Fe ₃ O ₄ -SiO ₂。此外，通过SEM、TEM、XRD、FT-IR和VSM对材料进行了表征和分析。最后，研究了固定化漆酶在极性/非极性/毒性体系中的热灭活动力学以及2,4-DCP的吸附和降解。结果表明漆酶@Fe ₃ O ₄ -SiO ₂在最佳条件下（pH 6，温度65°C，2,4-DCP初始浓度10 mg/L），去除率高达81.6%。此外，与游离漆酶相比，固定化漆酶在低pH和高温条件下具有良好的耐受性，储存稳定性也有很大提高。_{Laccase@Fe 3} O ₄ -SiO ₂重复使用7次后仍能保持59%的2,4-DCP去除率，具有工业应用潜力。