ABSTRACT

Pollution of aquatic bodies by pharmaceutical compounds is an emerging environmental problem because these drugs are extensively used in animal and human medicine. Ibuprofen, 2-[4-(2-methylpropyl)phenyl] propanoic acid, is the non-steroidal anti-inflammatory drug mainly present both in wastewater and in rivers and lakes in Europe. Despite this environmental emergency is giving rise a great interest in the scientific community, literature about the effects of ibuprofen on microalgae is scarce. A previous work on the freshwater microalga Scenedesmus rubescens had shown some morpho-physiological effects caused by treatment with 62.5, 250 and 1000 µg L⁻¹ ibuprofen. In this paper, we presented the results of treatment with ibuprofen at the same concentrations, ranging from environmental levels up to higher values, on Chlamydomonas reinhardtii (P.J.L. Dangeard). Ibuprofen effects on the alga were assayed through analyses of growth curve. Moreover, analyses of cell morphology, ultrastructure, and photosynthetic pigments were performed. The first effect of drug was the stimulation of the microalga growth. In addition, following exposure to ibuprofen, cells exhibited morphological and ultrastructural alterations, the latter ones consisting of starch granules and both in vacuolar and cytoplasmatic inclusions. On the contrary, chlorophyll and carotenoids amounts, were not affected by drug treatment.

摘要

药物化合物对水体的污染是一个正在出现的环境问题，因为这些药物被广泛用于动物和人类医学中。布洛芬是2- [4-（2-甲基丙基）苯基]丙酸，是一种非甾体类抗炎药，主要存在于欧洲的废水以及河流和湖泊中。尽管这种环境紧急情况引起了科学界的极大兴趣，但是关于布洛芬对微藻的影响的文献却很少。先前对淡水微藻景物的研究表明，用62.5、250和1000 µg L ^-1处理可引起一定的形态生理效应。布洛芬。在本文中，我们介绍了在莱茵衣藻（PJL Dangeard）上使用相同浓度的布洛芬治疗的结果，浓度范围从环境水平到较高值。通过分析生长曲线来测定布洛芬对藻类的作用。此外，进行了细胞形态，超微结构和光合色素的分析。药物的第一个作用是刺激微藻生长。另外，暴露于布洛芬后，细胞表现出形态和超微结构改变，后者由淀粉颗粒以及液泡和细胞质内含物组成。相反，叶绿素和类胡萝卜素的量不受药物处理的影响。