Shrimp fisheries are among the most important fisheries worldwide, and shrimp culture has increased considerably in recent years. Most current studies on reproduction-related genes have been conducted on cultured shrimp. However, gene expression is intimately linked to physiological and environmental conditions, and therefore an organism’s growth environment has a great influence on reproduction. Thus, gene expression profiling, should be applied in fisheries studies. Here, we identified the expression patterns of 76 reproduction-related genes in P. vannamei via the analysis of pooled transcriptomes from a time-series experiment encompassing a full circadian cycle. The expression patterns of genes associated both directly (Vtg, ODP, and ProR) and indirectly (FAMet, CruA1, and CruC1) with reproduction were evaluated, as these genes could be used as molecular markers of previtellogenic and vitellogenic maturation stages. The evaluated genes were prominently upregulated during vitellogenic stages, with specific expression patterns depending on the organism’s environment, diet, and season. Vtg, ProR, ODP, and FaMet could serve as molecular markers for both wild and cultured organisms.

中文翻译：

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养殖和野生太平洋白对虾（Penaeus vannamei）卵黄形成的比较：下一代测序和与繁殖直接和间接相关的基因的相对表达

虾类渔业是世界上最重要的渔业之一，近年来，虾类养殖已大大增加。目前对繁殖相关基因的大多数研究都是在养殖虾上进行的。然而，基因表达与生理和环境条件密切相关，因此生物的生长环境对繁殖有很大影响。因此，基因表达谱分析应在渔业研究中应用。在这里，我们通过对来自包含整个昼夜节律周期的时序实验的合并转录组的分析，确定了南美白对虾中76个繁殖相关基因的表达模式。评价了与繁殖直接相关（Vtg，ODP和ProR）和间接（FAMet，CruA1和CruC1）相关的基因的表达模式，因为这些基因可用作玻璃体形成和卵黄形成成熟阶段的分子标记。被评估的基因在卵黄形成阶段明显上调，其具体表达方式取决于生物体的环境，饮食和季节。Vtg，ProR，ODP和FaMet可以作为野生和培养生物的分子标记。