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Identification of long noncoding RNAs in silkworm larvae infected with Bombyx mori cypovirus
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-02-22 , DOI: 10.1002/arch.21777 Zhendong Zhang 1, 2 , Ze Zhao 1, 2 , Su Lin 1, 2 , Wanming Wu 1, 2 , Weiming Tang 1, 2 , Youfu Dong 1, 2 , Manman Shen 1, 2 , Ping Wu 1, 2 , Xijie Guo 1, 2
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-02-22 , DOI: 10.1002/arch.21777 Zhendong Zhang 1, 2 , Ze Zhao 1, 2 , Su Lin 1, 2 , Wanming Wu 1, 2 , Weiming Tang 1, 2 , Youfu Dong 1, 2 , Manman Shen 1, 2 , Ping Wu 1, 2 , Xijie Guo 1, 2
Affiliation
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Bombyx mori cypovirus (BmCPV) is one of the most important pathogens causing severe disease to silkworm. Emerging evidence indicates that long noncoding RNAs (lncRNAs) play importantly regulatory roles in virus infection and host immune response. To better understand the interaction between silkworm, Bombyx mori and BmCPV, we performed a comparative transcriptome analysis on lncRNAs and mRNAs between the virus‐infected and noninfected silkworm larvae midgut at two time points postinoculation. A total of 16,753 genes and 1845 candidate lncRNAs were identified, among which 356 messenger RNA (mRNAs) and 41 lncRNAs were differentially expressed (DE). Target gene prediction revealed that most of DEmRNAs (123) were coexpressed with 28 DElncRNAs, suggesting that the expression of mRNA is mainly affected through trans‐ regulation by BmCPV‐induced lncRNAs, and a regulatory network of DElncRNAs and DEmRNAs was then constructed. According to the network, many genes involved in apoptosis, autophagy, and antiviral response, such as ATG3, PDCD6, IBP2, and MFB1, could be targeted by different DElncRNAs, implying the essential roles of these genes and lncRNAs in BmCPV infection. In all, our studies revealed for the first time the alteration of lncRNA expression in BmCPV‐infected larvae and its potential influence on BmCPV replication, providing a new perspective for host–cypovirus interaction studies.
中文翻译：
家蚕丝状杯状病毒感染的家蚕幼虫中长非编码RNA的鉴定
家蚕丝状病毒（BmCPV）是引起家蚕严重疾病的最重要的病原体之一。新兴证据表明，长的非编码RNA（lncRNA）在病毒感染和宿主免疫反应中起着重要的调节作用。为了更好地了解家蚕之间的相互作用和BmCPV，我们在接种后两个时间点对病毒感染和未感染的蚕幼虫中肠的lncRNA和mRNA进行了转录组比较分析。共鉴定到16,753个基因和1845个候选lncRNA，其中356个信使RNA（mRNA）和41个lncRNA被差异表达（DE）。靶基因预测显示，大多数DEmRNA（123）与28种DElncRNA共表达，这表明mRNA的表达主要受反式表达的影响。通过BmCPV诱导的lncRNA进行调控，然后构建DElncRNA和DEmRNA的调控网络。根据网络，许多涉及凋亡，自噬和抗病毒反应的基因，例如ATG3，PDCD6，IBP2和MFB1，可能会被不同的DElncRNA靶向，这暗示这些基因和lncRNA在BmCPV感染中起着至关重要的作用。总之，我们的研究首次揭示了感染BmCPV的幼虫中lncRNA表达的改变及其对BmCPV复制的潜在影响，为宿主-杯状病毒相互作用研究提供了新的视角。
更新日期：2021-03-11
中文翻译：
家蚕丝状杯状病毒感染的家蚕幼虫中长非编码RNA的鉴定
家蚕丝状病毒（BmCPV）是引起家蚕严重疾病的最重要的病原体之一。新兴证据表明，长的非编码RNA（lncRNA）在病毒感染和宿主免疫反应中起着重要的调节作用。为了更好地了解家蚕之间的相互作用和BmCPV，我们在接种后两个时间点对病毒感染和未感染的蚕幼虫中肠的lncRNA和mRNA进行了转录组比较分析。共鉴定到16,753个基因和1845个候选lncRNA，其中356个信使RNA（mRNA）和41个lncRNA被差异表达（DE）。靶基因预测显示，大多数DEmRNA（123）与28种DElncRNA共表达，这表明mRNA的表达主要受反式表达的影响。通过BmCPV诱导的lncRNA进行调控，然后构建DElncRNA和DEmRNA的调控网络。根据网络，许多涉及凋亡，自噬和抗病毒反应的基因，例如ATG3，PDCD6，IBP2和MFB1，可能会被不同的DElncRNA靶向，这暗示这些基因和lncRNA在BmCPV感染中起着至关重要的作用。总之，我们的研究首次揭示了感染BmCPV的幼虫中lncRNA表达的改变及其对BmCPV复制的潜在影响，为宿主-杯状病毒相互作用研究提供了新的视角。
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