Fluorinated fentanyl regioisomers have become increasingly common in forensic casework. This study reports the synthesis of eight fluorinated fentanyl derivatives as pure reference materials and their complete NMR, infrared and mass spectral characterisation. Presumptive colour tests and TLC results are presented as an initial screening method for these compounds. The fully validated GC–MS method (employing SIM mode) allows the quantification of the target analytes (LOD = 9–20 ng/mL, LOQ = 31–67 ng/mL), fentanyl, heroin, acetaminophen and caffeine, within 13 min. In most cases, the regioisomeric fluorofentanyls were resolved from each other except for the 3′- and 4′-fluorinated derivatives (5b and 5c). To achieve full separation, an orthogonal benchtop ¹⁹F NMR method allows the identification and quantification of target analogues (LOD = 74–400 μg/mL, LOQ = 290–1340 μg/mL). The ¹⁹F NMR method allows the detection of fluorinated fentanyl analogues at a low concentration (2.4% w/w) in heroin.

在法医案件中，氟化的芬太尼区域异构体变得越来越普遍。这项研究报告了八种氟化芬太尼衍生物的合成，它们是纯净的参考材料，并且具有完整的NMR，红外和质谱表征。推定的颜色测试和TLC结果是这些化合物的初步筛选方法。经过充分验证的GC-MS方法（采用SIM模式）可以在13分钟内定量分析目标分析物（LOD = 9–20 ng / mL，LOQ = 31–67 ng / mL），芬太尼，海洛因，对乙酰氨基酚和咖啡因。在大多数情况下，除了3'-和4'-氟化衍生物（5b和5c）外，区域异构的氟芬太尼彼此分开。为了实现完全分离，正交台式¹⁹F NMR方法可以鉴定和定量目标类似物（LOD = 74–400μg/ mL，LOQ = 290–1340μg/ mL）。的¹⁹ F NMR方法允许以低浓度（2.4％的氟化类似物芬太尼的检测w / w的海洛因）。