We have prepared and characterized a cholesterol-rich nanoemulsion called LDE, a mimic of classic lipoprotein macromolecules, that can be applied as a new drug delivery system for aluminum phthalocyanine chloride (PcAlCl). The LDE containing PcAlCl system prepared herein had mean size and zeta potential of 127 nm and −29 mV, respectively, and encapsulation rate efficiency was 81%, and stability of 17 months. Compared to classical liposomes, LDE was more efficient, especially in brain diseases like glioblastoma (GBM), as revealed by tests on the U-87 MG cell line. The LDEPc formulation did not display dark cytotoxicity, as expected. The best light dose for LDEPc was 1.0 J·cm⁻²: its activity was 55% higher than PcAlCl in a compatible organic medium. In the U-87 MG cells, apoptosis was the preferential pathway activated by PDT. These results strongly support the use of LDE as a new theranostic system.

我们已经制备并表征了一种富含胆固醇的纳米乳液，称为LDE，它是经典脂蛋白大分子的模拟物，可以用作铝酞菁氯化物（PcAlCl）的新药物递送系统。本文制备的含LDE的PcAlCl系统的平均大小和Zeta电位分别为127 nm和-29 mV，包封率效率为81％，稳定性为17个月。与传统脂质体相比，LDE更有效，特别是在脑胶质母细胞瘤（GBM）等脑部疾病中，如对U-87 MG细胞系进行的测试所揭示的那样。如预期的那样，LDEPc制剂未显示出暗细胞毒性。LDEPc的最佳光剂量为1.0 J·cm ^-2：在相容的有机介质中，其活性比PcAlCl高55％。在U-87 MG细胞中，凋亡是PDT激活的优先途径。这些结果有力地支持了将LDE用作新的治疗诊断系统。