Due to restriction of the use of BPA, several structural analogues such as BPS and BPF have been proposed for its replacement in many consumer products. This has increased the prevalence of BPS and BPF in urine from tested cohorts. However, these substitutes have similar endocrine disrupting properties to BPA, particularly on reproductive and metabolic functions, which suggests that fetal exposure to these analogues could be of concern for human health. Bisphenols (BPs) are mainly metabolized to glucuronides (BP-Gs), which are considered as inactive but provide a relevant marker of fetal exposure during pregnancy. In most instances, these metabolites are indirectly quantified after hydrolysis and measurement of the corresponding native BPs, which may lead to bias due to spurious BPs contamination during blood collection and/or analyses. We have developed a new method for direct quantification of BP-Gs, which has the advantage of not being affected by errors related to the presence of BPs. First, BP-Gs were extracted from plasma by anion exchange solid phase extraction. They were then labelled with dansyl chloride, using experimentally-optimized incubation conditions, after which the dansyl derivatives were injected into an on-line SPE-UHPLC/MS/MS system. The performance of the method, in terms of sensitivity, precision and accuracy, was evaluated in plasma over a concentration range of 0.05–5 ng/mL. The intra- and inter-day CV% precision were lower than 20% with accuracies ranging from 93% to 115%. The limit of quantification was set at 0.05 ng/mL. The method was then applied to measure BP-Gs in forty-four cord plasma samples. Although no BPF-G was found, BPA-G and BPS-G was determined in almost half of the cord plasma samples with concentration ranges nd-0.089 ng/mL and nd-0.586 ng/mL, respectively.

由于限制了BPA的使用，有人提出了一些结构类似物（例如BPS和BPF）来替代许多消费产品。这增加了测试人群的尿液中BPS和BPF的患病率。然而，这些替代物具有与BPA相似的内分泌干扰特性，特别是在生殖和代谢功能方面，这表明胎儿暴露于这些类似物可能会影响人类健康。双酚（BPs）主要代谢为葡糖醛酸（BP-Gs），后者被认为是无活性的，但提供了怀孕期间胎儿暴露的相关标志。在大多数情况下，这些代谢物在水解和测量相应的天然BP后会间接定量，这可能会因血液采集和/或分析过程中的BP伪造污染而导致偏差。我们已经开发了一种直接定量BP-G的新方法，其优点是不受与BP的存在有关的误差的影响。首先，通过阴离子交换固相萃取从血浆中萃取BP-Gs。然后，使用实验优化的孵育条件，将它们用丹磺酰氯标记，然后将丹磺酰衍生物注入在线SPE-UHPLC / MS / MS系统中。该方法在灵敏度，精密度和准确性方面的性能在0.05-5 ng / mL的浓度范围内的血浆中进行了评估。日内和日间CV％精度低于20％，准确度在93％至115％之间。定量限设定为0.05 ng / mL。然后将该方法用于测量四十四条脐带血浆样品中的BP-Gs。虽然没有发现BPF-G，