The hatchability, mortality rate, lipid peroxide levels, and swimming speed of Artemia salina have been compared based on short exposures of ZnCl₂, CdCl₂, and HgCl₂ in artificial seawater. The hatching tests were carried out for 12, 24, 36, and 48 h at 28 °C. Mortality rate and lipid peroxide (LPO) levels were determined after 24 h of exposure at 28 °C, in the dark, and on living larvae using the FOX method. The swimming speed was determined after 24 h using a microcomputer coupled to a digital camera, with simultaneous treatment of the recorded images every 25 s, at 25 °C, under red-light irradiation. Results showed that Zn caused a gradual inhibition of the hatching for concentrations <900 µmol L⁻¹; however, Cd and Hg displayed almost complete inhibition for concentrations ≤100 µmol L⁻¹. Also, the heavy metals caused a dose-dependent increase of mortality (LD50) in the following order: Zn = 3290 µmol L⁻¹ < Cd = 2206 µmol L⁻¹ < Hg = 15.6 µmol L^−1. Furthermore, significant LPO levels were found for Cd (1500–2000 µmol L⁻¹, p < 0.001) and Hg (5–20 µmol L⁻¹, p < 0.001). Finally, the swimming speed values increased significantly, for Zn ≈ 2.5 mm s⁻¹ (1500 µmol L⁻¹, p < 0.001), Cd ≈ 3.5 mm s⁻¹ (2000 µmol L⁻¹, p < 0.05), and Hg ≈ 4.0 mm s⁻¹ (15 µmol L⁻¹, p < 0.05), after 24 h exposure. There is a clear dose-dependent toxicity, indicating that Zn, Cd and Hg can induce significant changes in hatchability, mortality, and ethological and biochemical parameters.

根据卤水在人工海水中的ZnCl ₂，CdCl ₂和HgCl _2的短时暴露，比较了卤虫的孵化率，死亡率，脂质过氧化物水平和游泳速度。孵化测试在28°C下进行了12、24、36和48小时。使用FOX方法，在28°C下暴露于黑暗中和活幼虫上24小时后，测定死亡率和过氧化脂质（LPO）水平。24小时后，使用连接至数码相机的微型计算机确定游泳速度，并在25°C下在红光照射下每25 s同时处理记录的图像。结果表明，浓度<900 µmol L ^-1时，Zn逐渐抑制了孵化; 但是，Cd和Hg对浓度≤100µmol L ^-1几乎显示出完全的抑制作用。此外，重金属导致死亡率的剂量依赖性增加（LD50），顺序为：Zn = 3290 µmol L ^-1 <Cd = 2206 µmol L ^-1 <Hg = 15.6 µmol L ^-1。此外，发现Cd（1500–2000 µmol L ^-1，p <0.001）和Hg（5–20 µmol L ^-1，p <0.001）的LPO水平很高。最后，对于Zn≈2.5 mm s ^-1（1500 µmol L ^-1，p <0.001），Cd≈3.5 mm s ^-1（2000 µmol L ^-1，p <0.05）和Hg ，游泳速度值显着增加≈4.0 mm s ^-1（15微摩尔L^-1，p <0.05），暴露24小时后。有明显的剂量依赖性毒性，表明锌，镉和汞可引起孵化率，死亡率以及行为学和生化参数的显着变化。