The Journal of Membrane Biology ( IF 2.3 ) Pub Date : 2021-02-23 , DOI: 10.1007/s00232-021-00175-0 Antje Pokorny 1 , Paulo F Almeida 1
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Abstract
The mechanism of the antimicrobial peptide daptomycin is reviewed and discussed. Daptomycin is a last-resort antibiotic in current use against drug-resistant bacterial infections. Many models have been proposed for its function, most based on the observation that it increases membrane permeability and causes leakage of contents, such as ions and small molecules from bacterial cells and lipid vesicles. However, daptomycin is actually not efficient at permeabilizing or translocating across membranes, contrary to many well-known antimicrobial peptides. There is strong evidence that daptomycin binds preferentially to membranes in active division regions of bacterial cells and that it causes large membrane reorganization in terms of the distribution of lipids and proteins, both in cells and in model membranes. Those observations support the alternative hypothesis for the mechanism of daptomycin that its primary effect is in inducing membrane reorganization and that other events, such as increased membrane leakage and depolarization, are secondary consequences, not essential to its function.
Graphic Abstract
中文翻译：
抗生素肽达托霉素通过重组膜发挥作用
摘要
对抗菌肽达托霉素的作用机制进行了综述和讨论。达托霉素是目前用于对抗耐药细菌感染的最后一种抗生素。已经针对其功能提出了许多模型，大多数基于观察到它增加膜通透性并导致内容物泄漏，例如来自细菌细胞和脂质囊泡的离子和小分子。然而，与许多众所周知的抗菌肽相反，达托霉素实际上在透化或跨膜转运方面效率不高。有强有力的证据表明，达托霉素优先与细菌细胞活跃分裂区域的膜结合，并且在细胞和模型膜中，就脂质和蛋白质的分布而言，它会导致大的膜重组。
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