The nominal volume of a variable volume micropipette is the maximum volume of the range specified by the micropipette manufacturer. The purpose of the present study is to quantify the reproducibility standard uncertainty for non-nominal volume micropipette calibrations for variable volume micropipettes. An interlaboratory study was implemented for this purpose, using variable volume micropipettes with nominal volumes of 10 μL, 200 μL, and 1000 μL and setting the measurement volumes as 10 %, 50 %, and 100 % of the nominal volumes. In our previous paper (Accredit Qual Assur 19:377, 2014), we quantified the uncertainty due to reproducibility using only data obtained for nominal volume calibrations. From the results obtained, we found that the reproducibility uncertainties quantified for nominal volumes are too small to cover interlaboratory variations for non-nominal volume calibrations. For calibrations of 10 % and 50 % of the nominal volumes, the reproducibility uncertainties must be increased by the factors of 2.7 and 1.4, respectively, for micropipettes with nominal volumes of 10 μL to 1 000 μL, based on the results of this study.

可变体积微量移液器的标称体积是微量移液器制造商指定范围内的最大体积。本研究的目的是量化可变体积微量移液器非标称体积微量移液器的重现性标准不确定度。为此目的进行了实验室间研究，使用标称体积为10μL，200μL和1000μL的可变体积微量移液器，并将测量体积设置为标称体积的10％，50％和100％。在我们之前的论文（Accredit Qual Assur 19：377，2014）中，我们仅使用名义体积校准获得的数据对由于可重复性造成的不确定性进行了量化。根据获得的结果，我们发现，对名义体积进行量化的可重复性不确定性太小，无法涵盖非名义体积校准的实验室间差异。根据本研究的结果，对于标称体积为10μL至1000μL的微量移液器，对于标称体积的10％和50％的校准，再现性不确定性必须分别增加2.7和1.4倍。