Nanotechnology is playing a major role in the field of medical diagnosis, in particular with the biosensor and bioimaging. It improves the performance of the desired system dramatically by displaying higher selectivity and sensitivity. Carbon nanomaterial, gold nanostructure, magnetite nanoparticle, and silica substrate are the most popular nanomaterials greatly contributed to make the affordable and effective biosensor at low-cost. This research work is introducing a new sensing strategy with graphene oxide-constructed triangular electrodes to diagnose Alzheimer’s disease (AD). MicroRNA-137 (miRNA-137) was found as a suitable biomarker for AD, and the sensing method was established here to detect miRNA-137 on the complementary sequence. To enhance the immobilization of capture miRNA-137, gold nanostar (GNS) was conjugated with capture miRNA and immobilized on the GO-modified surface through an amine linker. This immobilization process enhanced the hybridization of the target and reaches the detection limit at 10 fM with the sensitivity of 1 fM on the linear curve with a regression coefficient of 0.9038. Further control sequences of miRNA-21 and single and triple base mismatched miRNA-137 did not show a significant response in current changes, indicating the specific miRNA-137 detection for diagnosing AD.

中文翻译：

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三角形电极上的氧化石墨烯-金星结构用于阿尔茨海默氏病的鉴定

纳米技术在医学诊断领域起着重要作用，尤其是在生物传感器和生物成像领域。通过显示更高的选择性和灵敏度，它可以显着提高所需系统的性能。碳纳米材料，金纳米结构，磁铁矿纳米颗粒和二氧化硅基质是最受欢迎的纳米材料，极大地有助于以低成本制造出价格合理且有效的生物传感器。这项研究工作正在引入一种新的传感策略，该方法采用氧化石墨烯构造的三角电极来诊断阿尔茨海默氏病（AD）。发现MicroRNA-137（miRNA-137）是AD的合适生物标志物，在此建立了检测方法以检测互补序列上的miRNA-137。为了增强捕获miRNA-137的固定化，将金纳米星（GNS）与捕获miRNA偶联，并通过胺接头固定在GO修饰的表面上。该固定化过程增强了靶标的杂交，并在10 fM时达到检测极限，线性曲线上的灵敏度为1 fM，回归系数为0.9038。miRNA-21和单碱基和三碱基错配的miRNA-137的其他控制序列在当前变化中未显示出明显的反应，这表明可检测出用于诊断AD的特异性miRNA-137。