RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3′ untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.

中文翻译：

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RNA结构集合的基因组尺度反卷积

当用化学探测查询 RNA 结构时，RNA 结构的异质性是一个主要挑战。我们介绍了 DRACO，这是一种从突变分析实验中解卷积共存 RNA 构象的算法。使用带有测序的硫酸二甲酯突变分析 (DMS-MaPseq) 和 DRACO 对 SARS-CoV-2 基因组进行分析，确定了折叠成两个互斥构象的多个区域，包括 3' 非翻译区域中的保守结构转换。这项工作可能为剖析 RNA 结构组的异质性开辟了道路。