Abstract. Dissolution of anthropogenic CO₂ is chronically acidifying aquatic ecosystems. Studies indicate that ocean acidification will cause marine life, especially calcifying species, to suffer at the organismal and ecosystem levels. In comparison, freshwater acidification has received less attention rendering its consequences unclear. Here, juvenile Chinese mitten crabs, Eriocheir sinensis, were used as a calcifying model to investigate the impacts of CO₂-mediated freshwater acidification. Our integrative approach investigating changes in the animal's acid-base homeostasis, metabolism, calcification, locomotory behaviour, and survival rate indicate that the crab will face energetic consequences from future freshwater acidification. These energetic trade-offs allow the animal to maintain its acid-base homeostasis at the cost of reduced metabolic activity, exoskeletal calcification, and locomotion reducing the animal's overall fitness and increasing its mortality. Results suggest that present-day calcifying invertebrates could be heavily affected by freshwater acidification similar to their marine organisms and emphasizes the importance in understanding the long-term implications of freshwater acidification on species fitness.

中文翻译：

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人为CO ₂介导的淡水酸化限制了耐胁迫的淡水甲壳类动物的存活，钙化，代谢和行为

摘要。人为CO _2的溶解正在长期酸化水生生态系统。研究表明，海洋酸化将导致海洋生物，尤其是钙化物种在生物和生态系统层面遭受损害。相比之下，淡水酸化受到的关注较少，其后果尚不清楚。在这里，使用中华绒螯蟹（Eriocheir sinensis）作为钙化模型来研究CO ₂的影响。介导的淡水酸化。我们的综合方法研究了动物的酸碱稳态，代谢，钙化，运动行为和存活率的变化，这些结果表明，螃蟹将面临未来淡水酸化带来的积极后果。这些有力的折衷使动物能够维持其酸碱稳态，但其代价是代谢活性降低，骨骼外钙化和运动减少了动物的整体健康状况并增加了死亡率。结果表明，当今的钙化无脊椎动物可能会像与其海洋生物一样受到淡水酸化的严重影响，并强调了理解淡水酸化对物种适应性的长期影响的重要性。