Abstract

Context

Type 2 diabetes mellitus (T2DM) has undeniably become a significant threat to public health in the modern world.

Objective

To identify molecules involved in the development of T2DM, the expression patterns of cis-lincRNAs-LEP were investigated in T2DM patients.

Materials and methods

The expression levels of 11 lncRNAs-LEP and LEP mRNA in 71 diabetic patients and 32 controls were determined using qRT-PCR. The association between lncRNAs-LEP and T2DM was performed using ROC curve analysis.

Results

The expression analysis of lnc-LEP-2:6 and lnc-LEP-2:7 was significantly different (p≤ .0001) in diabetic patients compared to the controls. The ROC curve data showed that lnc-LEP-2:6 and lnc-LEP-2:7 have a high AUC, with 0.940 (95% CI: 0.875–0.977) and 0.958 (95% CI: 0.899–0.988), respectively.

Conclusions

Our results revealed two novel biomarkers associated with the T2DM and suggest that the circulating intergenic, lnc-LEP-2:6 and lnc-LEP-2:7, might have a regulatory role in the disease pathogenesis.

中文翻译：

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基因间 lnc-LEP-2:6 和 lnc-LEP-2:7 作为与 2 型糖尿病相关的新型生物标志物

摘要

语境

不可否认，2 型糖尿病 (T2DM) 已成为现代世界公共健康的重大威胁。

客观的

为了鉴定参与 T2DM 发展的分子，研究了 T2DM 患者中 cis-lincRNAs-LEP 的表达模式。

材料和方法

使用 qRT-PCR 测定 71 名糖尿病患者和 32 名对照者中11 种 lncRNA-LEP 和LEP mRNA的表达水平。使用 ROC 曲线分析进行 lncRNAs-LEP 和 T2DM 之间的关联。

结果

与对照组相比，糖尿病患者中lnc-LEP-2:6 和 lnc-LEP-2:7 的表达分析显着不同 ( p ≤ .0001)。ROC曲线数据显示，lnc-LEP-2:6和lnc-LEP-2:7具有较高的AUC，分别为0.940（95% CI：0.875-0.977）和0.958（95% CI：0.899-0.988） 。

结论

我们的结果揭示了与 T2DM 相关的两个新生物标志物，并表明循环基因间 lnc-LEP-2:6 和 lnc-LEP-2:7 可能在疾病发病机制中具有调节作用。