Hard fescue (Festuca brevipila Tracey; 2n = 6x = 42) is a cool season turfgrass with a fine leaf texture that performs well under low-input management. Breeding and genetics studies of F. brevipila have been limited due to the complexity of its hexaploid genome. To advance our knowledge of F. brevipila genomics, we used PacBio isoform sequencing to develop a reference transcriptome for this species. Here we report the F. brevipila reference transcriptome generated from root, crown, leaf, and inflorescence tissues. We obtained 59,510 full-length transcripts, of which 38,556 were nonredundant full-length transcripts. The longest and shortest transcripts were 11,487 and 58 bp, respectively. Distribution of synonymous distances among paralogs within F. brevipila suggested highly similar subgenomes that are difficult to distinguish from sequencing errors. To evaluate the phylogenetic relationships among F. brevipila and close relatives, we sequenced three additional transcriptomes using closely related species on an Illumina platform. The results of our phylotranscriptomic analysis supported the close relationships among F. brevipila (6x), Festuca ovina (4x), Festuca ovina subsp. ovina (2x), and Festuca valesiaca (2x), with high levels of discordance among gene trees. Overall, the F. brevipila PacBio Isoseq reference transcriptome provides the foundation for transcriptome studies and offers breeders a resource for gene discovery in this important turfgrass species.

中文翻译：

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使用 PacBio Isoseq 和 Illumina 测序的组合为六倍体硬羊茅草皮草 (Festuca brevipila) 构建参考转录组

硬羊茅 ( Festuca brevipila Tracey; 2 n  = 6 x  = 42) 是一种凉爽的季节草坪草，叶片质地细腻，在低投入管理下表现良好。由于其六倍体基因组的复杂性，短叶镰刀菌的育种和遗传学研究受到限制。为了提高我们对短叶镰刀菌基因组学的了解，我们使用 PacBio 同种型测序来开发该物种的参考转录组。在这里我们报告F. brevipila从根、冠、叶和花序组织生成的参考转录组。我们获得了 59,510 个全长转录本，其中 38,556 个是非冗余全长转录本。最长和最短的转录本分别为 11,487 和 58 bp。F. brevipila中旁系同源物之间同义距离的分布表明，亚基因组高度相似，难以与测序错误区分开来。为了评估F. brevipila和近亲之间的系统发育关系，我们在 Illumina 平台上使用密切相关的物种对三个额外的转录组进行了测序。我们的系统转录组学分析的结果支持F. brevipila (6 x )之间的密切关系，Festuca ovina (4 x ), Festuca ovina subsp。ovina (2 x ) 和Festuca valesiaca (2 x )，基因树之间存在高度不一致。总的来说，F. brevipila PacBio Isoseq 参考转录组为转录组研究奠定了基础，并为育种者提供了在这一重要草坪草物种中进行基因发现的资源。