Canine mammary gland tumour (CMTs) are one of the most commonly found tumours in intact female dogs. A previous study on canine mammary glands demonstrated the presence of the transient receptor potential melastatin 7 (TRPM7) ion channels in healthy canine mammary tissues. However, the significance of TRPM7 in CMT is not yet known. TRPM7 is a Ca²⁺ and Mg²⁺ permeable cation channel that contains a protein kinase domain. The aim of this study was to determine TRPM7 expression in 57 benign and malignant CMT tissues of dogs using immunohistochemistry (IHC) and evaluate its correlation with clinicopathological features and explore the potential prognostic value of TRPM7 in a prospective survival study. IHC analysis shows that TRPM7 was expressed in the cytoplasm of neoplastic epithelial cells. Moreover, TRPM7 expression was significantly associated with tumour malignancy (P = .027), Ki-67 index (P < .0001) and metastasis (P < .0001). Survival curve analysis indicates that high TRPM7 expression was significantly associated with poor disease-free (P = .035) and overall survival (P = .011) in malignant CMTs. Our results demonstrate that TRPM7 is expressed in CMTs and that its expression is positively correlated with clinicopathological parameters. Thus, TRPM7 was assumed to be a potential prognostic factor for CMTs.

中文翻译：

---

TRPM7在犬乳腺肿瘤中的表达及预后价值

犬乳腺肿瘤 (CMT) 是完整雌性犬中最常见的肿瘤之一。先前对犬乳腺的研究表明，在健康的犬乳腺组织中存在瞬时受体电位 melastatin 7 (TRPM7) 离子通道。然而，TRPM7 在 CMT 中的意义尚不清楚。TRPM7 是 Ca ²⁺和 Mg ²⁺含有蛋白激酶结构域的可渗透阳离子通道。本研究的目的是使用免疫组织化学 (IHC) 确定犬的 57 种良性和恶性 CMT 组织中 TRPM7 的表达，并评估其与临床病理学特征的相关性，并探索 TRPM7 在前瞻性生存研究中的潜在预后价值。IHC 分析显示 TRPM7 在肿瘤上皮细胞的细胞质中表达。此外，TRPM7 表达与肿瘤恶性程度 ( P  = .027)、Ki-67 指数 ( P  < .0001) 和转移 ( P  < .0001) 显着相关。生存曲线分析表明，高 TRPM7 表达与较差的无病（P  = .035）和总生存期显着相关（P  = .011) 在恶性 CMT 中。我们的结果表明，TRPM7 在 CMT 中表达，并且其表达与临床病理参数呈正相关。因此，TRPM7 被认为是 CMT 的潜在预后因素。