Kwan et al. (2017) published an informative study comparing results obtained by next‐generation sequencing (NGS) of mean bacterial genera richness among different life stages, male and female adults, and rearing conditions (field vs. laboratory) for Ixodes pacificus. The current paper examines Kwan et al. (2017) as a case study to provide guidance on statistical design and analysis for estimation of richness, derived from next generation sequencing technology, of the bacterial microbiome in field‐collected I. pacificus. Suggestions are provided to further strengthen quantification of microbiome richness in studies in ticks, with focus on sampling design. In‐depth treatment is provided of the relative merits of estimating mean richness versus median richness. Research on microbiome diversity in ticks can be made quantitatively rigorous; although, more research on methods is needed.

中文翻译：

---

tick中细菌丰富度的严格定量：一个案例研究

关等。（2017）发表了一项有益的研究，比较了通过下一代测序（NGS）获得的不同物种在不同生命阶段，成年男女之间的平均细菌属丰富度，以及太平洋硬x的饲养条件（田间还是实验室）。目前的论文对关等人进行了研究。（2017年）作为一个案例研究，提供统计设计和丰富的估算分析指导，从下一代测序技术中的源细菌微生物的，现场采集的我。太平洋。提供了一些建议，以进一步加强壁虱研究中微生物组丰富度的量化，重点是抽样设计。提供了对估计平均富裕度与中位数富裕度的相对优点的深入处理。壁虱中微生物组多样性的研究可以进行严格的定量研究。但是，需要对方法进行更多的研究。