Despite the economic and ecological importance of Hydnum species, in vitro synthesis of ectomycorrhizae of this genus has not been reported due to difficulties in establishing pure cultures. We inoculated pure cultures of 12 monokaryotic and 3 dikaryotic mycelial strains of an undescribed Hydnum albidum-like species on roots of axenic Pinus densiflora seedlings to synthesize ectomycorrhizae and to evaluate their mycorrhiza-forming ability. Six months after inoculation, both monokaryotic and dikaryotic strains formed ectomycorrhizae with Hartig net hyphae at the root cortex. Monokaryotic and dikaryotic strains exhibited similar morpho-anatomical characteristics of ectomycorrhizae, with the exception for clamped septa of emanating and outer mantle hyphae in the latter. Between monokaryotic and descendant dikaryotic strains, there were no significant differences in number of mycorrhizae in pine seedlings, whereas monokaryotic strains showed a greater total number of root tips and lower colonization rates than the descendant dikaryotic strains. These results indicate that both monokaryotic and dikaryotic mycelia of the H. albidum-like species can form mycorrhizae under axenic condition, and that can be applied toward the cultivation of hedgehog mushrooms.

尽管绣球属物种在经济和生态方面具有重要意义，但由于难以建立纯培养物，因此尚未报道该属的外生菌根的体外合成。我们在树胶松树的根部接种了未描述的类似Hydnum albidum的12个单核和3个双核菌丝体菌株的纯培养物。幼苗合成外生菌根并评估其菌根形成能力。接种六个月后，单核和双核菌株均在根皮层形成带有哈蒂格净菌丝的外生菌根。单核和双核菌株表现出相似的外生菌根的形态解剖学特征，除了后者的发散和外层菌丝的固定隔片。在单核和后代双核菌株之间，松树幼苗中的菌根数没有显着差异，而单核型菌株显示出比后代双核菌株更大的根尖总数和更低的定植率。这些结果表明，两个单核和dikaryotic菌丝体H. albidum样物种可能在无菌条件下形成菌根，可用于种植刺猬蘑菇。