A new wild type solventogenic Clostridium bacterium, which could produce a large amount of solvent from a mixture of 70% glucose and 30% xylose, was isolated. Based on 16S rRNA gene analysis, this strain was identified as Clostridium beijerinckii. Batch fermentations with this strain (C. beijerinckii GSC1) resulted in the production of 19.65 g/L total solvents (Acetone-Butanol-Ethanol), which is 31% higher than that with the typical wild type strain Clostridium acetobutylicum ATCC 824. This new strain utilized glucose and xylose simultaneously without genetic modification. The selectivity of GSC1 for butanol was 81.8%, which is much higher than that of C. acetobutylicum ATCC 824 (68.7%). Simple genetic modification was performed to obtain a more improved performance. The acid production in batch fermentation by C. beijerinckii GSC1_R1 (gene-modified strain) was reduced to 2.6 g/L from 6.02 g/L. The solvent productivity of GSC1_R1 in continuous fermentation was 3.6 g/L/h. These results indicate that the newly isolated strain is very promising and applicable for the production of biobutanol from second-generation biomass owing to the superior performance of the wild type strain.

分离了一种新的野生型产溶剂梭状芽胞杆菌细菌，该细菌可从70％葡萄糖和30％木糖的混合物中产生大量溶剂。基于16S rRNA基因分析，该菌株被鉴定为拜氏梭菌。使用该菌株（C. beijerinckii GSC1）进行分批发酵，产生的总溶剂量为19.65 g / L（丙酮-丁醇-乙醇），比典型的野生型菌株丙酮丁醇梭菌ATCC 824高31％。该菌株同时利用葡萄糖和木糖而不进行基因修饰。GSC1对丁醇的选择性为81.8％，远高于丙酮丁醇梭菌的丁醇。ATCC 824（68.7％）。进行简单的基因修饰以获得更好的性能。拜氏梭菌GSC1_R1（基因修饰菌株）在分批发酵中的产酸从6.02 g / L降低到2.6 g / L。连续发酵中GSC1_R1的溶剂生产率为3.6 g / L / h。这些结果表明，由于野生型菌株的优异性能，新分离出的菌株非常有前途并且可用于由第二代生物质生产生物丁醇。