We report the laser-ablative fabrication of antibacterial nanostructures, combining mechanical and chemotoxic effects, by femto- and nanosecond laser ablation and their testing on Staphylococcus aureus and Pseudomonas aeruginosa strains. A Ti surface, covered with nanospikes and periodical surface ripples, provides a strong anti-biofilm effect, and the addition of cytotoxic nanoparticles (NPs) (Ag, Se) enhances the overall bactericidal activities. A flow reactor, including Ti nanospike-covered wafers as a through-flow sterilizer, allowed reduction of the bacterial population by two orders. Several types of nanostructures with/without biofilms were characterized by scanning electron microscopy and energy-dispersive x-ray spectroscopy. Their antibacterial properties were demonstrated by standard live/dead tests and were suggested to originate from the combined chemical toxicity of NPs and mechanical damage caused by sharp nanoscale relief on Ti.

我们报告了飞秒和纳秒激光消融及其对金黄色葡萄球菌和铜绿假单胞菌的测试，结合了机械和化学毒性作用，对激光纳米结构进行了激光烧蚀加工。株。覆盖有纳米钉和周期性表面波纹的Ti表面具有很强的抗生物膜作用，并且添加了细胞毒性纳米颗粒（NPs）（Ag，Se）可以增强总体杀菌活性。包括钛纳米钉覆盖的晶圆作为流通式灭菌器的流式反应器使细菌数量减少了两个数量级。具有/不具有生物膜的几种类型的纳米结构通过扫描电子显微镜和能量色散X射线光谱学表征。通过标准活/死试验证明了它们的抗菌性能，并认为它们的产生是由于NP的化学毒性和Ti的纳米级纳米级起伏引起的机械损伤的综合作用。