Abstract

Johne’s disease is a chronic, contagious, zoonotic disease that affects numerous species including livestock and sometimes humans. The disease is globally distributed in sheep populations and caused by Mycobacterium avium Subsp. paratuberculosis (MAP). A previous genome-wide association study identified single nucleotide polymorphism (SNP) markers associated with OJD serostatus in CD109, PCP4, and SEMA3D genes. Our aim was to evaluate the same markers for association with OJD seroprevalence in Turkish sheep in a retrospective matched case-control study. The serological status for OJD in 1801 sheep was determined for four native and four composite breeds from three research flocks. One hundred eleven matched case-control pairs were constructed according to breed type and age from 1750 comingled ewes reared in the same environment. A Single Nucleotide Primer Extension (SNuPE) assay was designed to genotype PCP4-Intron 1, PCP4-3’UTR, SEMA3D, CD109-intron 2 and CD109-intron 8 markers and a McNemar’s test was performed on the matched pairs. An association with these five markers was not detected with the OJD serostatus in Turkish sheep (power of detection, 0.95; odds ratio >3; McNemar’s p < .05). Thus, a wider search may be needed to identify any major underlying genetic risk factors for OJD in Turkish sheep.

摘要

约内氏病是一种慢性、传染性、人畜共患疾病，影响许多物种，包括牲畜，有时也影响人类。这种疾病在全球范围内分布在绵羊与所造成鸟分枝杆菌亚种。副结核病(MAP)。之前的一项全基因组关联研究确定了与CD109、PCP4和SEMA3D 中OJD 血清状态相关的单核苷酸多态性 (SNP) 标记基因。我们的目的是在一项回顾性匹配病例对照研究中评估与土耳其绵羊 OJD 血清阳性率相关的相同标志物。1801 只绵羊 OJD 的血清学状态由来自三个研究羊群的四个本地品种和四个复合品种确定。根据品种类型和年龄从 1750 只在相同环境中饲养的混合母羊构建了 111 对匹配的病例对照对。单核苷酸引物延伸 (SNuPE) 分析设计用于对PCP4 -内含子 1、PCP4 -3'UTR、SEMA3D、CD109 -内含子 2 和CD109进行基因分型-内含子 8 标记和 McNemar 测试对匹配的配对进行。土耳其绵羊的 OJD 血清状态未检测到与这五个标记的关联（检测能力，0.95；优势比 >3；McNemar's p  < .05）。因此，可能需要更广泛的搜索来确定土耳其绵羊 OJD 的任何主要潜在遗传风险因素。