A case of multi‐male spawning between two males and one female of Rhaebo guttatus is reported. An amplectant pair of R. guttatus was observed spawning in an ephemeral puddle located in a flooded area of a stream inside an open ombrophilous forest remnant on 31st January 2019, in the municipality of Cotriguaçu, north‐western Mato Grosso state, Brazil. To our surprise, a conspecific male (secondary male) was submerged with its belly up, nostrils out of the water and clasping the female by the ventral region, apparently, trying to fertilise the eggs. For Neotropical anurans, multi‐male spawning has been reported to occur in eight phyllomedusid and four leptodactylid species. Therefore, it is the first record of conspecific multi‐male spawning for R. guttatus. These observations provide new insights into the breeding biology of R. guttatus. Further studies, however, are needed to better understand the reproductive features, focusing on genetic analyses of the offspring produced from multi‐male spawning of R. guttatus to determine whether multiple paternity occurs in this toad species.

中文翻译：

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三是人群：在斑蟾蜍Rhaebo guttatus中繁殖的同种多雄性（Schneider，1799）

据报道，有一个多雄性产卵在Rhaebo guttatus的两个雄性和一个雌性之间。一个amplectant对R.鱯观察到位于流的洪泛区在2019年1月31日开放ombrophilous森林遗迹内的短暂水坑产卵，在科特里瓜苏，西北部的马托格罗索州，巴西的直辖市。令我们惊讶的是，一个同种的雄性（次生雄性）腹部被淹没，鼻孔从水中浸出，并在腹侧区域紧紧抓住雌性，显然是在试图使卵子受精。据报道，对于新热带无脊椎动物，在八种叶状纲和四种l形纲中都发生了多雄性产卵。因此，这是条形杜鹃R. guttatus的同种多雄性产卵的第一条记录。。这些观察提供了新的洞察力guttatus繁殖生物学的见解。然而，需要进一步的研究来更好地理解生殖特征，重点是对多齿雄性古塔罗非鱼产卵后代的遗传分析，以确定在这种蟾蜍物种中是否存在多重亲子关系。