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Characterization and enhanced extracellular overexpression of a new salt-activated alginate lyase
Journal of the Science of Food and Agriculture ( IF 3.3 ) Pub Date : 2021-02-19 , DOI: 10.1002/jsfa.11161
Qing Meng 1 , Xinyu Tian 1 , Bo Jiang 1, 2 , Licheng Zhou 1 , Jingjing Chen 1 , Tao Zhang 1
Affiliation  

Alginate lyases (EC 4.4.2.3/4.4.2.11) have been applied to produce alginate oligosaccharides, which have physiological advantages such as prebiotic and antidiabetic effects, and are of benefit in the food and pharmaceutical industries. Extracellular production of recombinant proteins in Escherichia coli presents advantages including simplified downstream processing and high productivity; however, the presence of certain signal peptides does not always ensure successful secretion, which make the extracellular production of alginate lyase in E. coli rarely reported but of great significance.

中文翻译:

一种新的盐活化藻酸盐裂解酶的表征和增强的细胞外过表达

海藻酸盐裂解酶(EC 4.4.2.3/4.4.2.11)已被应用于生产海藻酸盐寡糖,其具有益生元和抗糖尿病作用等生理优势,在食品和制药行业中受益。大肠杆菌中重组蛋白的胞外生产具有简化下游加工和高生产率等优点;然而,某些信号肽的存在并不总能确保成功分泌,这使得在大肠杆菌中藻酸盐裂解酶的胞外产生鲜有报道但具有重要意义。
更新日期:2021-02-19
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