Aspongopus chinensis Dallas, 1851 is an important insect resource with a long utilization history as traditional Chinese medicine and food owing to its various health benefits, including anti-cancer, anti-bacteria, and anti-clotting properties. However, the long period of reproductive diapause during the overwintering stage has limited the broad utilization and artificial cultivation of A. chinensis. Diapause is largely regulated by juvenile hormones. Therefore, understanding the relationship between juvenile hormone metabolism and A. chinensis diapause may provide useful insight for developing genetic engineering strategies to regulate diapause. We identified differentially expressed genes in diapause and non-diapause adults of A. chinensis by transcriptome sequencing. A total of 336,230,260 clean reads were assembled into 80,769 unigenes. Overall, 3,524 differentially expressed genes were identified, including 2,174 down-regulated and 1,350 up-regulated genes in diapause adults. Among these differentially expressed genes, 22 were significantly enriched in the JH III metabolism-related pathway based on Kyoto Encyclopedia of Genes and Genomes analysis. These results provide insight into the molecular-level mechanism of diapause regulation and highlight new targets for preventing diapause to improve A. chinensis cultivation and productivity.

达拉斯Aspongopus chinensis达拉斯，1851年作为重要的昆虫资源，由于具有多种抗癌，抗菌和抗凝血特性，因此作为中药和食品具有很长的使用历史，是中药和食品的重要来源。但是，越冬期繁殖滞育的长期性限制了中华曲霉的广泛利用和人工栽培。滞育在很大程度上受少年激素的调节。因此，了解少年激素代谢和中华稻滞育之间的关系可能为开发调控滞育的基因工程策略提供有用的见解。我们鉴定了中华稻滞育和非滞育成虫的差异表达基因通过转录组测序。总共336,230,260个干净读段被组装成80,769个单基因。总体而言，在滞育成年成年人中，鉴定出3,524个差异表达基因，包括2,174个下调基因和1,350个上调基因。在这些差异表达基因中，根据《京都基因百科全书》和《基因组学》分析，有22种显着丰富了JH III代谢相关途径。这些结果为深入了解滞育调控的分子水平机制提供了见识，并突出了预防滞育的新目标，从而提高了中华稻的栽培和生产力。