Chitosan is drawing a lot of attention and starts to play a significant role in the sustainability of aquaculture. It meets the environmental criteria, which is an eco-friendly compound, helps the efficient use of reagents and reduction of possible waste as well. The current study was conducted to evaluate the effect of dietary supplements of chitosan (CS) and chitosan nanoparticles (CNP) at a level of 0.5 % on two different based diets on Nile tilapia health and innate immune responses. The experimental design was completely randomized with three replicates in a 2 × 3 factorial design; with two different based diets; FM (fishmeal-based diet) and GM (Gluten meal-based diet); with three forms of chitosan (zero-chitosan as a control, CS and CNP). chitosan form and dietary protein source significantly influenced the hematological indices, phagocytic activity and index, while RBCs, Hct, MCV, WBCs and Phagocytic activity were also significantly affected by both factors interaction (P < 0.05). Moreover, the antioxidant defense system activities and biochemical parameters were significantly affected (P < 0.05) by dietary protein source, chitosan form, and their interaction. Fish fed on GM diet recorded a remarkable reduction (P < 0.05) in immunity parameters, antioxidant system activities and the biochemical indices among all treatments. Conversely, fish fed on plant-base diet (GM) and received chitosan fortification especially CNP, recorded a significant enhancement (P < 0.05) in health status. Therefore, the present study recommends the addition of supplementary CNP to the Nile tilapia diet for better health and disease resistance, as well as augmentation of plant-based protein nutritional value.

壳聚糖受到了广泛关注，并开始在水产养殖业的可持续性中发挥重要作用。它符合环保标准，是一种环保化合物，有助于有效使用试剂并减少可能的浪费。进行本研究以评估0.5％的壳聚糖（CS）和壳聚糖纳米颗粒（CNP）膳食补充剂对两种不同日粮对尼罗罗非鱼健康和先天免疫反应的影响。实验设计在2×3因子设计中完全随机重复了三个重复。有两种不同的饮食习惯；FM（以鱼粉为主的饮食）和GM（以谷粉为主的饮食）；具有三种形式的壳聚糖（零壳聚糖作为对照，CS和CNP）。壳聚糖的形式和膳食蛋白质来源显着影响血液学指标，吞噬活性和指数，而RBC，Hct，MCV，WBC和吞噬活性也受到两个因素相互作用的显着影响（P <0.05）。此外，膳食蛋白质来源，壳聚糖形式及其相互作用对抗氧化防御系统的活性和生化参数有显着影响（P <0.05）。在所有处理中，以转基因饮食喂养的鱼的免疫参数，抗氧化系统活性和生化指标均显着降低（P <0.05）。相反，以植物性饮食（GM）为食并接受壳聚糖强化（尤其是CNP）的鱼的健康状况显着增强（P <0.05）。因此，本研究建议在尼罗罗非鱼饮食中添加补充CNP，以改善健康状况和抗病能力，