The present research aimed to investigate the protection of N-acetylcysteine (NAC) on T-2 toxin-induced toxicity in Chinese mitten crab (Eriocheir sinensis). Crabs (0.27 ± 0.00 g) were fed four different diets (Control, T-2, 0.05% NAC + T-2 and 0.1% NAC + T-2) for 8 weeks. The results showed that T-2 toxin decreased growth performance, and induced oxidative stress, immunodepression and apoptosis in juvenile crabs. Crabs with NAC had higher weight gain, specific growth rate and hepatosomatic index than those in the T-2 group. The 0.1% NAC elevated crab survival and oxygen consumption rate and decreased alanine transaminase and aspartate transaminase in the serum of crabs fed T-2 toxin. Reactive oxygen species and malonaldehyde levels of hemolymph and hepatopancreas in crabs fed NAC were significantly lower than those in the T-2 group, while glutathione peroxidase, total antioxidant capacity, γ-glutamylcysteine synthetase activities and glutathione content significantly increased with 0.1% NAC. Total hemocyte count, respiratory burst activity, total protein, acid phosphatase (ACP) in hemolymph and ACP, alkaline phosphatase (AKP) in hepatopancreas were significantly enhanced with 0.1% NAC. Phagocytosis and AKP in hemolymph were significantly enhanced with 0.05% NAC supplementation. Furthermore, the mRNA expressions of antimicrobial peptides (ALF1, ALF2, crustin-1 and crustin-2) and peroxinectin were up-regulated with NAC. Besides NAC down-regulated the mRNA expressions of Relish, lipopolysaccharide-induced TNF-alpha factor, caspase-3, caspase-8 and p53 genes, and the ratio of Bax to Bcl-2 in the hepatopancreas. Our findings indicate that dietary NAC could improve the growth performance and survival of juvenile Chinese mitten crab fed T-2 toxin. The 0.1% NAC could effectively alleviate oxidative stress, immunodepression and apoptosis induced by T-2 toxin.

本研究旨在探讨N-乙酰半胱氨酸（NAC）对中华绒螯蟹（Eriocheir sinensis）T-2毒素诱导的毒性的保护作用）。螃蟹（0.27±0.00 g）被喂食四种不同的饮食（对照组，T-2、0.05％NAC + T-2和0.1％NAC + T-2）8周。结果表明，T-2毒素降低了幼蟹的生长性能，并诱导其氧化应激，免疫抑制和细胞凋亡。与T-2组相比，带有NAC的螃蟹的增重，比生长速率和肝体指数更高。0.1％NAC可以提高喂食T-2毒素的螃蟹血清中螃蟹的存活率和耗氧率，并降低丙氨酸转氨酶和天冬氨酸转氨酶。用NAC喂养的蟹的活性氧和血淋巴细胞和肝胰腺丙二醛的含量显着低于T-2组，而谷胱甘肽过氧化物酶，总抗氧化能力，γ-谷氨酰半胱氨酸合成酶活性和谷胱甘肽含量随0.1％NAC显着增加。0.1％NAC可显着提高总血细胞计数，呼吸爆发活性，总蛋白，血淋巴中的酸性磷酸酶（ACP）和肝胰腺中的碱性磷酸酶（AKP）。补充0.05％NAC可使血淋巴中的吞噬作用和AKP显着增强。此外，抗菌肽的mRNA表达（NAC会上调ALF1，ALF2，Crustin-1和Crustin-2和过氧化物酶的表达。除了NAC，肝胰脏中Relish，脂多糖诱导的TNF-α因子，caspase-3，caspase-8和p53基因的mRNA表达以及Bax与Bcl-2的比率均下调。我们的发现表明，日粮NAC可以改善饲喂T-2毒素的中华绒螯蟹的生长性能和存活率。0.1％NAC可以有效减轻T-2毒素诱导的氧化应激，免疫抑制和细胞凋亡。