Root rot leads to a substantial decline in the production and quality of soybean (Glycine max), particularly in continuous cropping systems. Therefore, it is important to explore methods and mechanisms for the biocontrol of root rot pathogens. In this paper, the fungal community structure in the roots and rhizosphere soil was determined by high-throughput sequencing technology under non-inoculated- and Funneliformis mosseae-inoculated conditions. Moreover, redundancy analysis (RDA) was used to analyse the effects of F. mosseae on the fungal community structure and the physical and chemical properties of the soil. In different samples (roots and rhizosphere soils), the fungal diversity index in HN48 was higher than that in HN66, while that without F. mosseae inoculation was slightly higher than that under inoculation with F. mosseae under different years of continuous cropping. Compared to those of non-inoculated plants, Fusarium oxysporum and Rhizoctonia solani were the dominant fungi in different root samples of soybean varieties. However, the predominant genera in the rhizosphere soil samples shifted with inoculation and comprised Fusarium, Mortierella, Cryptococcus, Guehomyces and Corynespora. Moreover, after inoculation with F. mosseae, the relative abundance of F. oxysporum and R. solani decreased in the roots and rhizosphere soils. Additionally, RDA showed that the rhizosphere soils showed significant differences depending on F. mosseae inoculation, continuous cropping year, and soybean variety. This study provides new insights into the interaction effects of arbuscular mycorrhizal fungi (AMF) and other fungi in continuously cropped soybean systems.

根腐病导致大豆（Glycine max）的产量和质量大幅下降，特别是在连作系统中。因此，探索根腐病菌的生物防治方法和机制具有重要意义。本文采用高通量测序技术，在非接种和漏斗状钉虫的条件下，确定了根和根际土壤中的真菌群落结构。此外，冗余度分析（RDA）被用来分析小菜蛾对真菌群落结构以及土壤理化性质的影响。在不同的样品（根和根际土壤）中，HN48的真菌多样性指数高于HN66的真菌多样性指数F.霉接种量略高于下与接种F.霉不同年份连作下。与未接种的植物相比，尖孢镰刀菌和茄根枯菌是大豆不同根样品中的优势真菌。然而，根际土壤样品中的主要属移接种和由镰孢属，被孢霉属，隐球菌属，Guehomyces和棒孢。此外，接种了F. mosseae之后，oxysporum和R. solani的相对丰度在根和根际土壤中减少。另外，RDA显示根际土壤显示出显着差异，这取决于F. mosseae接种，连作年和大豆品种。这项研究提供了对丛生大豆系统中丛枝菌根真菌（AMF）和其他真菌相互作用的新见解。