Abstract

This article contains an analysis of structural and functional parameters of bacterial complexes taken for examination from complete profiles of fens assigned to the Klyukvennoe and Karbyshevskoe wetlands in Tomsk oblast. Bacteria in the fens are revealed throughout the entire profile. Their number determined by a direct method of fluorescence microscopy is high and varies from 27 to 78 × 10⁹ cells/g of dry peat. The high number of saprotrophic bacteria (to 10⁸–10⁹ CFU/g of dry peat) is detected by the plate method. An increase in the number of saprotrophic bacteria down the fen profile is revealed. These data enable us to conclude for the first time that index k (a dimensionless universal characteristic of natural prokaryotic communities) in fen profiles decreases with depth. The bacterial cultures isolated from the fens studied are assigned to ten taxa: Bacillus, Streptomyces, Arthrobacter, Microbacterium, Pseudomonas, Flavobacterium, Dyadobacter, Janthinobacterium, Pedobacter, and Myxobacteriales. The acrotelm is dominated by bacterial cultures represented by genera Bacillus and Streptomyces; and Pseudomonas, Dyadobacter, and Arthrobacter are the dominants of the catotelm. The functions of bacterial genera and species identified by molecular biological methods are described. The study of nitrogen fixation, denitrification, and methanogenesis has shown that bacteria are viable and active throughout the entire peat profile.

中文翻译：

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托木斯克州真菌的细菌复合物：丰度，分类结构，活动

摘要

本文包含对细菌复合物的结构和功能参数的分析，以从托木斯克州Klyukvennoe和Karbyshevskoe湿地的类的完整概况中进行检查。中的细菌在整个轮廓中都可以看到。通过直接的荧光显微镜法测定的它们的数目很高，并且从27至78×10 ⁹细胞/克干泥炭变化。腐生细菌数量众多（至10 ⁸ –10 ⁹通过平板法检测CFU /克干泥炭。揭示了在腐状态下腐生细菌的数量增加。这些数据使我们首次得出结论：剖面中的指数k（自然原核生物群落的无量纲普遍特征）随深度减小。从研究的沼泽中分离的细菌培养物被分配到10类群：芽孢杆菌属，链霉菌属，节杆菌，微杆菌，假单胞菌属，黄杆菌属，Dyadobacter，Janthinobacterium，Pedobacter和Myxobacteriales。顶肢以芽孢杆菌和链霉菌属为代表的细菌培养; 和假单胞菌，Dyadobacter和节是catotelm的优势种群。描述了通过分子生物学方法鉴定的细菌属和种的功能。对固氮，反硝化和产甲烷作用的研究表明，细菌在整个泥炭剖面中都具有活力和活性。