Both clear cell renal carcinoma (ccRCC) and clear cell carcinoma of the ovary (CCOC) have a clear cytoplasmic morphological feature, hence it is difficult to identify metastatic ccRCC and CCOC by morphology alone. At present, there are no effective immunohistochemical markers to distinguish between these two tumors. Studies have shown that the clear cytoplasm of ccRCC is mainly caused by cholesterol-rich lipids in the cytoplasm, while that of CCOC is due to the accumulation of cytoplasmic glycogen. Objective: to hypothesize that the scavenger receptor class B-type 1 (SR-B1) protein responsible for HDL cholesterol uptake may be differentially expressed in ccRCC and CCOC, and high CD10 expression in the renal tubular epithelium may assist in distinguishing between ccRCC and CCOC. Methods: effective immunohistochemical markers were applied in 90 cases of renal clear cell carcinoma and 31 cases of ovarian cancer to distinguish between the two types of tumors.Result: SR-B1 and CD10 expression is significantly higher in ccRCC than CCOC. Both SR-B1 and CD10 exhibited focal weak-medium intensity staining in CCOC, and their staining extent and intensity were significantly lower than ccRCC. The sensitivity and specificity of SR-B1 for identifying ccRCC were 74.4% and 83.9%, respectively. The sensitivity and specificity of CD10 for identifying CCOC were 93.3% and 80.6%, respectively. The combined SR-B1( +) CD10( +) immunoprofile supports the diagnosis of ccRCC with a specificity of 93.5%. The combined SR-B1(-) CD10(-) immunoprofile supports the diagnosis of CCOC with a specificity of 93.3%. Conclusions: our findings demonstrate that the combination of SR-B1 and CD10 immunoprofiling is a valuable tool for differential diagnosis of ccRCC and CCOC.

透明细胞肾癌（ccRCC）和卵巢透明细胞癌（CCOC）均具有明确的细胞质形态学特征，因此仅通过形态学很难识别转移性ccRCC和CCOC。目前，尚无有效的免疫组化标记物来区分这两种肿瘤。研究表明，ccRCC的细胞质清澈主要是由于细胞质中富含胆固醇的脂质造成的，而CCOC的细胞质清澈则是由于细胞质糖原的积累所致。目的：假设负责 HDL 胆固醇摄取的清道夫受体 B 型 1 (SR-B1) 蛋白在 ccRCC 和 CCOC 中可能存在差异表达，肾小管上皮中高 CD10 表达可能有助于区分 ccRCC 和 CCOC 。方法：应用有效的免疫组化标记物对90例肾透明细胞癌和31例卵巢癌进行区分，以区分两类肿瘤。结果：ccRCC中SR-B1和CD10的表达显着高于CCOC。 SR-B1和CD10在CCOC中均呈现局灶性弱中强度染色，其染色程度和强度均显着低于ccRCC。 SR-B1 识别 ccRCC 的敏感性和特异性分别为 74.4% 和 83.9%。 CD10识别CCOC的敏感性和特异性分别为93.3%和80.6%。联合 SR-B1(+)CD10(+) 免疫谱支持 ccRCC 的诊断，特异性为 93.5%。联合 SR-B1(-) CD10(-) 免疫谱支持 CCOC 的诊断，特异性为 93.3%。结论：我们的研究结果表明，SR-B1 和 CD10 免疫分析相结合是鉴别诊断 ccRCC 和 CCOC 的有价值的工具。