Many studies to date on the response of cherry flower buds to frost have focused on visual evaluations by observing tissue browning after frost event in the orchard and laboratory conditions but only little knowledge is available on the consequences of intracellular ice formation on cellular ultrastructure that underlies exothermic reactions during bud burst stages. In this study, the differential thermal analysis (DTA) method was used to investigate critical frost temperatures for the sweet cherry cultivars '0900-Ziraat', 'Erzincan Macar', 'Lambert', 'Vista', 'Stella', and 'Early Burlat' under laboratory-based freeze assays. In the course of our experimental study, frost tolerance or cell death points (CDPs) of flowers of six cherry cultivars were investigated in consecutive phenological stages from the start to the end of blooming, for 2 years. The frost tolerance of flower buds changed according to different developmental stages and cherry cultivars. Our results of frost tolerance tests performed on the cherry blooming stages are rather controversial. Our findings have shown that at the open cluster stage, the frost tolerance of the flower buds is very sensitive (mCDP = −1.18°C for 'Lambert'), while the first white stage has revealed an important increase (mCDP= −9.96°C for '0900-Ziraat') in the frost tolerance of those. Averaged over 2 years, the temperatures causing 50% frost damage for flower buds were −2.08 to −3.76°C at the side green stage, −1.49 to −3.22°C at the green tip stage, −1.18 to −1.98°C at the open cluster stage, −7.92 to −9.96°C at the first white stage, and −6.29 to −9.36°C at the full bloom stage in the range of six cultivars. Based on our test results, '0900-Ziraat' and 'Vista' were regularly classified as frost-tolerant cultivars. The flower buds of 'Lambert' and 'Early Burlat' have been regularly the most sensitive, while 'Erzincan Macar' and 'Stella' were ranked into the group of medium sensitivity. These results can help farmers to estimate possible frost damages on sweet cherry flower buds due to frost events at the investigated phenological stages.

迄今为止，关于樱桃花蕾对霜冻的反应的许多研究都集中在视觉评估上，方法是观察果园和实验室条件下霜冻事件后组织褐变，但对细胞内冰的形成对放热的基础上细胞超微结构的后果了解甚少。在芽爆发阶段的反应 在这项研究中，使用差热分析（​​DTA）方法研究了甜樱桃品种'0900-Ziraat'，'Erzincan Macar'，'Lambert'，'Vista'，'Stella'和'Early'的临界霜冻温度。 Burlat'在基于实验室的冷冻测定中。在我们的实验研究过程中，从开花的开始到结束，在连续的物候阶段研究了六个樱桃品种的花的抗冻性或细胞死亡点（CDP），为期两年。花蕾的抗冻性根据不同的发育阶段和樱桃品种而变化。我们在樱花开花期进行的耐霜性测试结果颇具争议。我们的发现表明，在开放簇生阶段，花蕾的抗冻性非常敏感（“ Lambert”的mCDP = -1.18°C），而白色的第一个阶段显示出重要的提高（mCDP = -9.96°）。 C为“ 0900-Ziraat”）的抗冻性。平均2年，对花蕾造成50％霜冻损害的温度在侧生阶段为-2.08至-3.76°C，在生梢阶段为-1.49至-3.22°C。在六个品种的范围内，开放簇生阶段为18至-1.98°C，第一白阶段为-7.92至-9.96°C，盛开期为-6.29至-9.36°C。根据我们的测试结果，“ 0900-Ziraat”和“ Vista”通常被归为耐霜品种。兰伯特（Lambert）和伯爵（Early Burlat）的花蕾通常是最敏感的，而埃尔金坎·马卡（Erzincan Macar）和斯特拉（Stella）的花蕾则被列为中等敏感度。这些结果可以帮助农民估计在调查的物候阶段由于霜冻事件而对甜樱桃花蕾造成的霜冻损害。“ Vista”和“ Vista”通常被归为耐霜品种。兰伯特（Lambert）和伯爵（Early Burlat）的花蕾通常是最敏感的，而埃尔金坎·马卡（Erzincan Macar）和斯特拉（Stella）的花蕾则被列为中等敏感度。这些结果可以帮助农民估计在调查的物候阶段由于霜冻事件而对甜樱桃花蕾造成的霜冻损害。“ Vista”和“ Vista”通常被归为耐霜品种。兰伯特（Lambert）和伯爵（Early Burlat）的花蕾通常是最敏感的，而埃尔金坎·马卡（Erzincan Macar）和斯特拉（Stella）的花蕾则被列为中等敏感度。这些结果可以帮助农民估计在调查的物候阶段由于霜冻事件而对甜樱桃花蕾造成的霜冻损害。