The Atlantic goliath grouper, Epinephelus itajara (Lichtenstein, 1822), is considered a critically endangered species. In Brazil, the harvesting, transportation, and sale of E. itajara or it’s by products have been prohibited, but illegal harvesting still occurs. This study developed a simple, sensitive and accurate procedure for the safe identification of E. itajara by polymerase chain reaction (PCR). A specific primer (16S-RYOP4) was designed, synthesized and grouped with universal primers. The efficiency of specific amplification of the new set of primers was proven using samples of E. itajara, four species of epinephelids and five other species of fish marketed in northern Brazil. This procedure can be used to inspect products sold in stores, supermarkets and fairs, especially in places of reduced scientific, technological and economic development, where there is a record of illegal fishing and selling of E. itajara.

大西洋巨人石斑鱼，Epinephelus itajara（利希滕斯坦，1822年），被认为是极度濒危的物种。在巴西，禁止捕捞，运输和销售大肠埃希氏菌或其副产品，但仍然发生非法收获。这项研究开发了一种简单，灵敏和准确的程序，用于通过聚合酶链反应（PCR）安全鉴定大黄麻黄。设计，合成了特异性引物（16S-RYOP4），并与通用引物分组。新的引物组特异性扩增的效率已通过伊塔哈拉肠杆菌的样品证明，巴西北部销售的4种of鱼和5种其他鱼类。此程序可用于检查在商店，超级市场和集市上出售的产品，尤其是在科学，技术和经济发展水平下降的地方，那里有非法捕捞和出售伊塔雷亚大肠杆菌的记录。