Bacterial canker and subsequent gummosis are caused by multiple pathogens and lead to significant yield and productivity losses in sweet cherry cultivation in Turkey. This study identified that Pseudomonas syringae pathovars were responsible for bacterial canker on sweet cherry orchards by using classical and molecular methods and evaluated the biocontrol effects of bacteriophages against P. syringae pv. syringae. Pathogenic bacteria were isolated from samples taken from plants showing symptoms of bacterial canker in cherry orchards located in İzmir and Manisa provinces. Specific pathogens were identified using pathogenicity, phenotypic tests, and simplex PCR. Bacteriophages effective against P. syringae strains were isolated from soil contaminated with pathogens identified in the diseased orchards using an optimized isolation protocol. The biocontrol activity of bacteriophage isolates against P. syringae pv. syringae was tested in vitro and in vivo. The results of pathogenicity tests on immature sweet cherry fruits and micropropagated cherry plantlets revealed 10 pathogenic bacteria isolates from 44 plant samples taken from sweet cherry orchards showing symptoms of bacterial canker. Ten isolates were identified as Pseudomonas syringae pv. syringae. Nine different pure bacteriophage isolates were effective. The results indicated that bacteriophage isolates may demonstrate variable reactivity against P. syringae pathovars.

中文翻译：

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评价丁香假单胞菌光伏的噬菌体。丁香从土耳其甜樱桃（李属avium L.）上的鳞茎中分离

细菌性溃疡病和随后的牙龈病是由多种病原体引起的，并导致土耳其甜樱桃种植中明显的产量和生产力损失。本研究通过经典和分子方法确定了丁香假单胞菌致病菌是导致甜樱桃园细菌枯萎的原因，并评估了噬菌体对丁香假单胞菌的生物防治作用。丁香科。从位于伊兹密尔和马尼萨省樱桃园的植物样本中分离出致病细菌，表现出细菌性溃疡病症状。使用致病性，表型测试和单纯PCR鉴定特定的病原体。使用优化的分离方案，从在患病果园中鉴定出的病原体污染的土壤中分离出对丁香假单胞菌菌株有效的噬菌体。噬菌体分离物对丁香假单胞菌PV的生防活性。在体外和体内测试了丁香科。对未成熟的甜樱桃果实和微繁殖的樱桃苗进行致病性测试的结果表明，从甜樱桃园采集的44种植物样品中分离出10种致病菌，显示出细菌性溃疡病症状。十个分离物被鉴定为丁香假单胞菌。丁香科。九种不同的纯噬菌体分离物是有效的。结果表明，噬菌体分离物可能表现出针对丁香假单胞菌病原体的可变反应性。对未成熟的甜樱桃果实和微繁殖的樱桃苗进行致病性测试的结果表明，从甜樱桃园采集的44种植物样品中分离出10种致病菌，显示出细菌性溃疡病症状。十个分离物被鉴定为丁香假单胞菌。丁香科。九种不同的纯噬菌体分离物是有效的。结果表明，噬菌体分离物可能表现出针对丁香假单胞菌病原体的可变反应性。对未成熟的甜樱桃果实和微繁殖的樱桃苗进行致病性测试的结果表明，从甜樱桃园采集的44种植物样品中分离出10种致病菌，显示出细菌性溃疡病症状。十个分离物被鉴定为丁香假单胞菌。丁香科。九种不同的纯噬菌体分离物是有效的。结果表明，噬菌体分离物可能表现出针对丁香假单胞菌病原体的可变反应性。