Advanced data processing and visualization techniques were applied to laser ablation-single particle-inductively coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag⁺). Highly multiplexed images with tissue level resolution were generated, showing the uptake and transformation of Ag⁺ to silver nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is predisposed by the reducing power of particular root compartments. Various Ag⁺ and AgNP visualization strategies are shown with special emphasis on spatially resolved information associated with the number and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained by combining the results of techniques from several complementary platforms.

中文翻译：

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激光消融-单颗粒-电感耦合等离子体质谱法作为纳米组学中的多模态生物成像工具

先进的数据处理和可视化技术已应用于从向日葵（Helianthus annuus L.）的根部成像获得的激光烧蚀-单颗粒电感耦合等离子体质谱（LA-sp-ICPMS）数据，这些离子暴露于离子银（Ag ⁺）。生成具有组织水平分辨率的高度多路复用图像，显示整个根部横截面中Ag ⁺的吸收和向银纳米颗粒（AgNPs）的转化，并揭示了生物合成的AgNPs的大小是由于特定根室的降低能力所致。各种银⁺展示了AgNP和AgNP可视化策略，并特别强调了与用户选择的根横截面区域中单个AgNP的数量和球等效尺寸相关的空间分辨信息，包括对AgNP分布的统计分析。通常，只能通过组合来自多个互补平台的技术结果来获得此多模态信息。